Fukumori F, Kudo T, Narahashi Y, Horikoshi K
J Gen Microbiol. 1986 Aug;132(8):2329-35. doi: 10.1099/00221287-132-8-2329.
The cellulase gene from the alkalophilic Bacillus sp. strain 1139 was cloned in Escherichia coli using pBR322. Plasmid pFK1 was isolated from transformants producing cellulase, and the cloned cellulase gene was found to be in a 4 X 6 kb HindIII fragment. The cellulase gene was subcloned in a functional state on a 2 X 9 kb DNA fragment and its nucleotide sequence was determined. The coding sequence showed an open reading frame encoding 800 amino acids. The pFK1-encoded cellulase had the same enzymic properties as the extracellular cellulase produced by the alkalophilic Bacillus sp. strain 1139, but its Mr was slightly higher.
使用pBR322在大肠杆菌中克隆了嗜碱芽孢杆菌1139菌株的纤维素酶基因。从产生纤维素酶的转化子中分离出质粒pFK1，发现克隆的纤维素酶基因位于一个4×6 kb的HindIII片段中。纤维素酶基因在一个2×9 kb的DNA片段上以功能状态亚克隆，并测定了其核苷酸序列。编码序列显示一个编码800个氨基酸的开放阅读框。pFK1编码的纤维素酶具有与嗜碱芽孢杆菌1139菌株产生的细胞外纤维素酶相同的酶学性质，但其相对分子质量略高。
