Mo Yunfei, Guo Rui, Zhang Yi, Xue Wei, Cheng Biao, Zhang Yuanming
1 Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University , Guangzhou, China .
2 Department of Biomedical Engineering, Jinan University , Guangzhou, China .
Tissue Eng Part A. 2017 Jul;23(13-14):597-608. doi: 10.1089/ten.tea.2016.0268. Epub 2017 Mar 27.
In this study, we successfully fabricated a novel drug and plasmid DNA (pDNA) dual delivery system by electrospinning the dispersion composed of polyethyleneimine-carboxymethyl chitosan/pDNA-angiogenin (ANG) nanoparticles, curcumin (Cur), poly (D, L-lactic-co-glycolic acid) (PLGA), and cellulose nanocrystals (CNCs). In vitro release studies showed that the bioactivity of Cur and ANG was preserved in the nanofibers, and a sequential release pattern was achieved in which nearly 90% of the Cur was released in ca. 6 days and the ANG release lasted up to about 20 days. In vitro cell culture results suggested that the composite nanofibers exhibit excellent biocompatibility. To evaluate the in vivo angiogenesis and anti-infection properties, the PLGA/CNC/Cur/pDNA-ANG composite nanofibers were transplanted into the infected full-thickness burn wounds. Biopsy specimens were harvested for histology, immunohistochemistry, immunofluorescence, real-time quantitative PCR, and Western blotting analyses. The results indicated that the PLGA/CNC/Cur/pDNA-ANG composite nanofibers not only prevented local infection but also promoted skin regeneration.
在本研究中，我们通过静电纺丝由聚乙烯亚胺-羧甲基壳聚糖/pDNA-血管生成素（ANG）纳米颗粒、姜黄素（Cur）、聚（D，L-丙交酯-乙交酯）（PLGA）和纤维素纳米晶体（CNC）组成的分散体，成功制备了一种新型的药物和质粒DNA（pDNA）双递送系统。体外释放研究表明，Cur和ANG的生物活性在纳米纤维中得以保留，并实现了一种顺序释放模式，其中近90%的Cur在约6天内释放，而ANG的释放持续约20天。体外细胞培养结果表明，复合纳米纤维表现出优异的生物相容性。为了评估体内血管生成和抗感染特性，将PLGA/CNC/Cur/pDNA-ANG复合纳米纤维移植到感染的全层烧伤创面。采集活检标本进行组织学、免疫组织化学、免疫荧光、实时定量PCR和蛋白质印迹分析。结果表明，PLGA/CNC/Cur/pDNA-ANG复合纳米纤维不仅预防了局部感染，还促进了皮肤再生。
