Lee Gahyun, Jeon Sunghoon, Lee Sang-Kwon, Cheon Byunggyu, Moon Sohyeon, Park Jun-Gyu, Cho Kyoung-Oh, Choi Jihye
College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea.
J Vet Sci. 2017 Dec 31;18(4):507-514. doi: 10.4142/jvs.2017.18.4.507.
This study evaluated whether renal perfusion changes can be noninvasively estimated by using contrast-enhanced ultrasonography (CEUS) in renal ischemia-reperfusion injury and investigated the correlation between renal perfusion measured by CEUS and necrosis and apoptosis of renal tubular epithelial cells. In six dogs with experimentally induced renal ischemia-reperfusion injury, changes in time to peak intensity, peak intensity, and area under the curve were measured on CEUS. Peak intensity and area under the curve of the renal cortex began to decrease on day 1 (about 20% lower than baseline) and reached the lowest levels (about 50% of baseline) on day 4. They then gradually increased until day 10, at which time peak intensity was about 87% and area under the curve was about 95% of baseline; neither fully recovered. Both parameters were strongly correlated with the necrosis scores on histopathologic examination on day 4 ( = -0.810 of peak intensity and = -0.886 of area under the curve). CEUS allowed quantitative evaluation of perfusion changes in acute renal ischemia-reperfusion injury, and CEUS results were correlated with renal tubular damage on histopathologic examination. Thus, CEUS could be a noninvasive, quantitative diagnostic method for determining progress of renal ischemia-reperfusion injury.
本研究评估了在肾缺血再灌注损伤中，是否可通过使用对比增强超声（CEUS）对肾灌注变化进行无创估计，并研究了CEUS测量的肾灌注与肾小管上皮细胞坏死及凋亡之间的相关性。在6只实验性诱导肾缺血再灌注损伤的犬中，在CEUS上测量了达峰强度时间、峰强度和曲线下面积的变化。肾皮质的峰强度和曲线下面积在第1天开始下降（比基线低约20%），并在第4天达到最低水平（约为基线的50%）。然后它们逐渐增加，直到第10天，此时峰强度约为基线的87%，曲线下面积约为基线的95%；均未完全恢复。这两个参数与第4天组织病理学检查的坏死评分均密切相关（峰强度的r = -0.810，曲线下面积的r = -0.886）。CEUS能够对急性肾缺血再灌注损伤中的灌注变化进行定量评估，且CEUS结果与组织病理学检查中的肾小管损伤相关。因此，CEUS可能是一种用于确定肾缺血再灌注损伤进展的无创定量诊断方法。
