用于检测病毒抗原抗体的抗生物素蛋白-生物素乳胶凝集试验。
Avidin-biotin latex agglutination assay for detection of antibodies to viral antigens.
作者信息
Shekarchi I C, Fuccillo D A, Sever J L, Madden D M
机构信息
Microbiological Associates, Inc., Life Sciences Center, Rockville, Maryland 20850.
出版信息
J Clin Microbiol. 1988 May;26(5):954-6. doi: 10.1128/jcm.26.5.954-956.1988.
A new method for attaching antigens to latex by an avidin-biotin technique is described. The procedure permits control of the amount of antigen attached to the latex and eliminates the need for highly purified antigens and destructive bridging chemicals. The avidin-biotin latex agglutination assay is a simple, rapid test well suited to detection of viral antibody. The sensitivity and specificity, respectively, of the avidin-biotin latex agglutination assay compared with other assay results for antibody to viruses were as follows: cytomegalovirus, 98 and 100% (indirect hemagglutination assay); measles virus, 96 and 100% (enzyme-linked immunosorbent assay); and herpes simplex virus, 78 and 100% (indirect hemagglutination assay).
本文描述了一种通过抗生物素蛋白-生物素技术将抗原附着于乳胶的新方法。该程序能够控制附着于乳胶的抗原量，并且无需高纯度抗原和具有破坏性的桥连化学物质。抗生物素蛋白-生物素乳胶凝集试验是一种简单、快速的检测方法，非常适合检测病毒抗体。与其他病毒抗体检测结果相比，抗生物素蛋白-生物素乳胶凝集试验的敏感性和特异性分别如下：巨细胞病毒，98%和100%（间接血凝试验）；麻疹病毒，96%和100%（酶联免疫吸附测定）；单纯疱疹病毒，78%和100%（间接血凝试验）。
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