用重组抗原乳胶凝集法检测人免疫缺陷病毒抗体
Detection of antibodies to human immunodeficiency virus by latex agglutination with recombinant antigen.
作者信息
Riggin C H, Beltz G A, Hung C H, Thorn R M, Marciani D J
机构信息
Cambridge BioScience Corporation, Worcester, Massachusetts 01605.
出版信息
J Clin Microbiol. 1987 Sep;25(9):1772-3. doi: 10.1128/jcm.25.9.1772-1773.1987.
Abstract
Recombinant human immunodeficiency virus (HIV) env antigen was attached to polystyrene particles, and these complexes were used to develop the first latex agglutination assay for antibodies to HIV. A total of 95 positive and 116 negative human serum samples were assayed for antibodies to HIV by latex agglutination, and results were compared with those of a commercial enzyme immunoassay. Latex agglutination was also compared with, and found to be completely concordant with, Western blot (immunoblot) analysis with virion antigens.
摘要
重组人免疫缺陷病毒（HIV）包膜抗原附着于聚苯乙烯颗粒上，这些复合物被用于开发首个检测HIV抗体的乳胶凝集试验。通过乳胶凝集试验对总共95份阳性和116份阴性人血清样本进行HIV抗体检测，并将结果与商业酶免疫测定结果进行比较。乳胶凝集试验还与使用病毒体抗原的蛋白质印迹（免疫印迹）分析进行了比较，结果发现两者完全一致。
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