College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China.
Department of Oral and Maxillofacial Surgery, First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin, PR China.
Carbohydr Polym. 2017 Jun 15;166:31-44. doi: 10.1016/j.carbpol.2017.02.059. Epub 2017 Feb 20.
In the present study, we developed a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide GRGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides. The hydrazone crosslinking strategy is simple, while circumventing toxicity, making this injectable system feasible, minimally invasive and easily translatable for regenerative purposes. By varying their weight ratios, the physicochemical properties of the mechanically stable hydrogel system were easily adjustable. Additionally, the preliminary studies demonstrated that chondrocyte behavior was dependent on HA/pectin composition and the presence of integrin binding moieties. Specifically, the incorporation of a certain amount of GRGDS oligopeptide into HA/pectin-based hydrogels could serve as a biologically active microenvironment that supported chondrocyte phenotype and facilitated chondrogenesis. Furthermore, the hydrogel system exhibited acceptable tissue compatibility by using a mouse subcutaneous implantation model. Overall, the novel injectable multicomponent hydrogel presented here is expected to be useful biomaterial scaffold for cartilage tissue regeneration.
在本研究中，我们开发了一种基于透明质酸-己二酰二肼和接枝寡肽 GRGDS 的氧化果胶的仿生可注射水凝胶系统，其中它们的酰腙衍生物能够实现多糖的共价酰腙交联。酰腙交联策略简单，同时避免了毒性，使这种可注射系统具有可行性、微创性和易于转化用于再生目的。通过改变它们的重量比，可以轻松调节机械稳定水凝胶系统的物理化学性质。此外，初步研究表明，软骨细胞的行为取决于 HA/果胶的组成和整联蛋白结合片段的存在。具体而言，将一定量的 GRGDS 寡肽掺入基于 HA/果胶的水凝胶中，可以作为支持软骨细胞表型和促进软骨生成的生物活性微环境。此外，该水凝胶系统通过使用小鼠皮下植入模型表现出可接受的组织相容性。总的来说，本文提出的新型可注射多组分水凝胶有望成为软骨组织再生的有用生物材料支架。
