Kurti S P, Emerson S R, Rosenkranz S K, Teeman C S, Emerson E M, Cull B J, Smith J R, Harms C A
Department of Kinesiology, Kansas State University, Manhattan, KS 66506, USA; Physical Activity and Nutrition-Clinical Research Consortium, Kansas State University, Manhattan, KS 66506, USA.
Physical Activity and Nutrition-Clinical Research Consortium, Kansas State University, Manhattan, KS 66506, USA; Department of Food, Nutrition, Dietetics and Health, Kansas State University, Manhattan, KS 66506, USA.
Nutr Res. 2017 Mar;39:61-68. doi: 10.1016/j.nutres.2017.02.003. Epub 2017 Feb 4.
A single high-fat meal (HFM) leads to an increase in triglycerides and oxidative stress. Oxidative stress can be assessed via 8-isoprostane generation, which is associated with the development of asthma and cardiovascular disease. No previous research has investigated whether airway and systemic 8-isoprostane increases postprandially in nonasthmatic participants according to the energy and fat content of a meal. Our purpose was to assess airway and systemic 8-isoprostane after a HFM and a true-to-life moderate-fat meal (MFM). We hypothesized that airway and systemic 8-isoprostane would increase after a HFM and a MFM, with the greatest increase in the HFM condition. Eight nonasthmatic men (25.8±6.9years) completed the HFM and MFM trials in a randomized crossover design. After a 10-hour fast, participants consumed either a HFM (71.13kJ/kg body mass, 60% fat, 23% CHO) or a MFM (35.56kJ/kg body mass, 30% fat, 52% CHO). Exhaled breath condensate to assess airway 8-isoprostane was collected at baseline and at 3 and 6hours postmeal. Venous blood samples were collected at baseline and hourly until 6hours postmeal to assess triglycerides, and every 3hours for systemic 8-isoprostane. Airway 8-isoprostane responses were not significant as a main effect of time (P=.072), between conditions (P=.365), or between time and condition (P=.319) postmeal. Systemic 8-isoprostane increased over time (P<.001), but not between conditions (P=.124) or between time and condition (P=.649) postmeal. Triglyceride incremental area under the curve was different in the HFM compared to the MFM condition (P=.013). After a HFM and a MFM, 8-isoprostane increases systemically; however, airway 8-isoprostane does not change.
一顿高脂餐（HFM）会导致甘油三酯和氧化应激增加。氧化应激可通过8-异前列腺素生成来评估，其与哮喘和心血管疾病的发生有关。此前尚无研究调查在非哮喘参与者中，气道和全身的8-异前列腺素是否会根据一餐的能量和脂肪含量在餐后增加。我们的目的是评估一顿高脂餐和一顿符合实际情况的中度脂肪餐（MFM）后气道和全身的8-异前列腺素水平。我们假设，一顿高脂餐和一顿中度脂肪餐后气道和全身的8-异前列腺素会增加，在高脂餐情况下增加幅度最大。八名非哮喘男性（25.8±6.9岁）采用随机交叉设计完成了高脂餐和中度脂肪餐试验。禁食10小时后，参与者食用一顿高脂餐（71.13kJ/千克体重，60%脂肪，23%碳水化合物）或一顿中度脂肪餐（35.56kJ/千克体重，30%脂肪，52%碳水化合物）。在基线以及餐后3小时和6小时收集呼出气冷凝物以评估气道8-异前列腺素。在基线和餐后每小时直至6小时采集静脉血样以评估甘油三酯，并每3小时采集一次用于评估全身8-异前列腺素。餐后气道8-异前列腺素反应作为时间的主要效应（P = 0.072）、不同餐食情况之间（P = 0.365）或时间与餐食情况之间（P = 0.319）均无显著差异。餐后全身8-异前列腺素随时间增加（P<0.001），但在不同餐食情况之间（P = 0.124）或时间与餐食情况之间（P = 0.649）无差异。与中度脂肪餐情况相比，高脂餐时甘油三酯曲线下增量面积不同（P = 0.013）。一顿高脂餐和一顿中度脂肪餐后，8-异前列腺素在全身增加；然而，气道8-异前列腺素没有变化。
