Huang Fengyi, Shen Xue, Zhang Yuzheng, Vuong Ann M, Yang Shuman
Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, China.
Department of Epidemiology and Biostatistics, School of Public Health, University of Nevada, Las Vegas, NV, United States.
Front Nutr. 2022 Sep 30;9:1007304. doi: 10.3389/fnut.2022.1007304. eCollection 2022.
Food consumption induces oxidative stress in humans, but the changes in oxidative stress levels after a regular meal are still unclear. We conducted an experimental study on 20 healthy volunteers (10 males, 10 females), who matched in age (±2 years). They were given a regular diet (total energy of 704 kcal, which contains 75 g of carbohydrates, 35 g of protein, and 29 g of lipids) at 11:30 a.m. after a fast of over 12 h. We collected 6-repeated measures of venous blood samples at 2-h intervals heparin anticoagulant tubes immediately after the meal (indicated as "0" h) and up to 10 h post-consumption. Biomarkers included plasma fluorescent products, plasma malondialdehyde, plasma total antioxidant capacity, and plasma superoxide dismutase. FlOPs were measured at three excitation/emission wavelengths (FlOP_320, FlOP_360, and FlOP_400). The average age and BMI for the twenty participants were 22.70 ± 1.98 years and 20.67 ± 2.34 kg/m, respectively. Within 10 h after the meal, the overall trend of FlOPs were generally similar. There was no evidence of dose response for any of the three FlOPs (all > 0.05). However, levels of MDA decreased with the time of fasting ( and < 0.05), with the biggest decrease occurring between 0 and 2 h post-meal. The overall trend of T-AOC and SOD levels also decreased with fasting time ( and < 0.05), though an increase was observed between 0 and 2 h following consumption. Levels of MDA, T-AOC, and SOD but not FlOPs, decreased with fasting time.
食物摄入会在人体中引发氧化应激，但正常饮食后氧化应激水平的变化仍不明确。我们对20名健康志愿者（10名男性，10名女性）进行了一项实验研究，他们的年龄匹配（±2岁）。在禁食超过12小时后的上午11:30，给他们提供了一顿常规饮食（总能量为704千卡，其中包含75克碳水化合物、35克蛋白质和29克脂质）。我们在餐后立即（标记为“0”小时）以及进食后长达10小时内，每隔2小时收集一次静脉血样本，共收集6次重复测量样本。生物标志物包括血浆荧光产物、血浆丙二醛、血浆总抗氧化能力和血浆超氧化物歧化酶。在三个激发/发射波长（FlOP_320、FlOP_360和FlOP_400）下测量荧光产物。这20名参与者的平均年龄和体重指数分别为22.70±1.98岁和20.67±2.34kg/m²。餐后10小时内，荧光产物的总体趋势大致相似。三种荧光产物中的任何一种都没有剂量反应的证据（均>0.05）。然而，丙二醛水平随禁食时间下降（P<0.05），最大降幅出现在餐后0至2小时之间。总抗氧化能力和超氧化物歧化酶水平的总体趋势也随禁食时间下降（P<0.05），不过在进食后0至2小时观察到有所增加。丙二醛、总抗氧化能力和超氧化物歧化酶水平随禁食时间下降，但荧光产物水平未下降。
