Tsuchiya Kazuyo, Ikeda Takuto, Batmunkh Baatarsuren, Choijookhuu Narantsog, Ishizaki Hidenobu, Hotokezaka Masayuki, Hishikawa Yoshitaka, Nanashima Atsushi
Department of Surgery, Faculty of Medicine, University of Miyazaki , 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan.
Department of Anatomy, Histochemistry and Cell Biology, Faculty of Medicine, University of Miyazaki , 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan.
Acta Histochem Cytochem. 2017 Feb 28;50(1):21-28. doi: 10.1267/ahc.16035. Epub 2017 Feb 22.
Mucosal immune dysregulation associated with T cells plays a critical role in the development of inflammatory bowel diseases (IBD). However, the definite significances of these cells in IBD still remain unclear. Therefore, we investigated the population and expression of CD4CD161 T cells in the colonic lamina propria mononuclear cells (LPMCs) in patients with IBD by analyses using flow cytometry and immunohistochemistry. Interleukin-10 (IL-10) mRNA levels in both LPMCs and CD4 T cells in lamina propria (LP-CD4 T cells) were measured using a real-time quantitative reverse transcription-polymerase chain reaction. IL-10 production was investigated with immunohistochemistry. The results revealed that the population of CD4CD161 T cells was significantly decreased in active ulcerative colitis (UC) compared with inactive UC ( < 0.05). The CD4CD161 T cell population was inversely correlated with disease activity in patients with UC (r = -0.6326, = 0.0055), but there was no significant correlation in those with Crohn's disease. Over-expression of IL-10 mRNA in both LPMCs and LP-CD4 T cells were detected in active UC. Immunohistochemistry revealed decreased frequency of CD161 cells and increased IL-10 positive cells in active UC. The frequency of CD4CD161 T cells and IL-10 expression was supposed to be associated with the pathological status of mucosal immunoregulation in IBD.
与T细胞相关的黏膜免疫失调在炎症性肠病（IBD）的发展中起关键作用。然而，这些细胞在IBD中的确切意义仍不清楚。因此，我们通过流式细胞术和免疫组织化学分析，研究了IBD患者结肠固有层单核细胞（LPMC）中CD4CD161 T细胞的数量和表达。使用实时定量逆转录-聚合酶链反应测量LPMC和固有层CD4 T细胞（LP-CD4 T细胞）中白细胞介素-10（IL-10）的mRNA水平。用免疫组织化学研究IL-10的产生。结果显示，与非活动性溃疡性结肠炎（UC）相比，活动性UC中CD4CD161 T细胞的数量显著减少（<0.05）。UC患者中CD4CD161 T细胞数量与疾病活动度呈负相关（r = -0.6326， = 0.0055），但克罗恩病患者中无显著相关性。在活动性UC中检测到LPMC和LP-CD4 T细胞中IL-10 mRNA均过表达。免疫组织化学显示，活动性UC中CD161细胞频率降低，IL-10阳性细胞增加。CD4CD161 T细胞频率和IL-10表达可能与IBD中黏膜免疫调节的病理状态相关。
