Benie Comoé Koffi Donatien, Dadié Adjéhi, Guessennd Nathalie, N'gbesso-Kouadio Nadège Ahou, Kouame N'zebo Désiré, N'golo David Coulibaly, Aka Solange, Dako Etienne, Dje Koffi Marcellin, Dosso Mireille
Laboratory of Biotechnology and Food Microbiology (LMBM), University of Nangui-Abrogoua, 02 BP 801, Abidjan 02, Côte d'Ivoire; Department of Bacteriology and Virology, Institut Pasteur of Côte d'Ivoire (IPCI), 01 BP 490, Abidjan 01, Côte d'Ivoire.
Department of Bacteriology and Virology, Institut Pasteur of Côte d'Ivoire (IPCI), 01 BP 490, Abidjan 01, Côte d'Ivoire; Faculty of Medical Sciences, University of Félix Houphouët Boigny, 01 BP V4, Abidjan 01, Côte d'Ivoire.
Eur J Microbiol Immunol (Bp). 2017 Feb 27;7(1):55-64. doi: 10.1556/1886.2016.00039. eCollection 2017 Mar.
owns a variability of virulence factors. These factors can increase bacterial pathogenicity and infection severity. Despite the importance of knowledge about them, these factors are not more characterized at level of strains derived from local food products. This study aimed to characterize the virulence potential of isolated from various animal products. Several structural and virulence genes of including , and were detected by polymerase chain reaction (PCR) on 204 strains of They were isolated from bovine meat (122), fresh fish (49), and smoked fish (33). The 16S rRNA gene was detected on 91.1% of the presumptive strains as The gene showed that 99.5% of the strains were . The gene (89.2%) was the most frequently detected ( < 0.05). In decreasing importance order, (86.8%), (72.1%), (72.1%), (40.2%), and (2.5%) were detected. The gene was detected in all strains of serogroups O11 and O16. The prevalence of , and genes in these strains varied from 51.2% to 87.4%. The simultaneous determination of serogroups and virulence factors is of interest for the efficacy of surveillance of infections associated with .
拥有多种毒力因子。这些因子可增加细菌致病性和感染严重程度。尽管了解它们很重要,但这些因子在源自当地食品的菌株水平上尚未得到更充分的表征。本研究旨在表征从各种动物产品中分离出的[细菌名称]的毒力潜力。通过聚合酶链反应(PCR)在204株[细菌名称]上检测了包括[具体基因名称1]、[具体基因名称2]和[具体基因名称3]在内的几种结构和毒力基因。它们分别从牛肉(122株)、鲜鱼(49株)和熏鱼(33株)中分离得到。在91.1%的推定菌株中检测到16S rRNA基因,为[具体结果]。[具体基因名称4]基因显示99.5%的菌株为[具体结果]。[具体基因名称5]基因(89.2%)是检测频率最高的(P<0.05)。按重要性递减顺序,检测到[具体基因名称6](86.8%)、[具体基因名称7](72.1%)、[具体基因名称8](72.1%)、[具体基因名称9](40.2%)和[具体基因名称10](2.5%)。在所有O11和O16血清型的[细菌名称]菌株中均检测到[具体基因名称11]基因。这些菌株中[具体基因名称12]、[具体基因名称13]和[具体基因名称14]基因的流行率在51.2%至87.4%之间。血清型和毒力因子的同时测定对于与[细菌名称]相关感染的监测效果具有重要意义。