Nasir Armanul, Rahman Shafkat Shamim, Hossain Md Mahboob, Choudhury Naiyyum
Department of Mathematics and Natural Sciences, BRAC University , Bangladesh.
Eur J Microbiol Immunol (Bp). 2017 Feb 27;7(1):76-91. doi: 10.1556/1886.2016.00035. eCollection 2017 Mar.
In view of the anticipated shortage of the traditional supplies of fossil fuels, there is a great deal of interest in the production of ethanol as an alternative biofuel in recent years. The main objective of this research work was to isolate and characterize stress tolerant, high potential ethanol producing yeast strains from various fruit peel. Two yeast isolates from pineapple (Pa) and orange (Or) have been isolated, characterized on the basis of morphological and physic-chemical characters and optimized on ethanol producing capability using sugarcane molasses as substrate. Ethanol production percentage was estimated by Conway method. Isolates were thermotolerant, pH tolerant, ethanol tolerant as well as osmotolerant. They were resistant to Chloramphenicol (30 μg/disc) and Nalidixic acid (30 μg/disc). The isolates showed no killer toxin activity against . The highest production capacity of the yeasts was found to be 7.39% and 5.02% for Pa and Or, respectively, at pH 5.0, 30 °C temperature in media with an initial reducing sugar concentration of 6.5% for Pa and 5.5% for Or (shaking). Addition of metal ions increased the rate of ethanol production highest to 10.61% by KHPO. This study revealed that indigenous yeast isolates could be used to benefit the fuel demand and industrial alcohol industries.
鉴于传统化石燃料供应预计短缺，近年来人们对生产乙醇作为替代生物燃料产生了浓厚兴趣。本研究工作的主要目的是从各种果皮中分离并鉴定出耐胁迫、具有高乙醇生产潜力的酵母菌株。已从菠萝（Pa）和橙子（Or）中分离出两株酵母菌株，根据形态学和物理化学特性进行了鉴定，并以甘蔗 molasses 为底物对其乙醇生产能力进行了优化。乙醇产量百分比通过 Conway 法估算。分离株具有耐热性、耐 pH 值、耐乙醇以及耐渗透压特性。它们对氯霉素（30 μg/圆盘）和萘啶酸（30 μg/圆盘）具有抗性。这些分离株对……未表现出杀伤毒素活性。在 pH 为 5.0、温度为 30°C 的培养基中，初始还原糖浓度为 Pa 的 6.5%和 Or 的 5.5%（振荡）时，酵母的最高生产能力分别为 Pa 的 7.39%和 Or 的 5.02%。添加金属离子使乙醇产量最高提高到 KHPO 的 10.61%。本研究表明，本地酵母分离株可用于满足燃料需求和工业酒精行业。
