Creaney Jenette, Dick Ian M, Leon Justine S, Robinson Bruce W S
National Centre for Asbestos Related Diseases, School of Medicine and Pharmacology, University of Western Australia and Australian Mesothelioma Tissue Bank, Sir Charles Gairdner Hospital, Perth, Western Australia
National Centre for Asbestos Related Diseases, School of Medicine and Pharmacology, University of Western Australia, Sir Charles Gairdner Hospital, Perth, Western Australia.
Cancer Genomics Proteomics. 2017 Mar-Apr;14(2):103-117. doi: 10.21873/cgp.20023.
Backgound/Aim: Malignant mesothelioma (MM) is an aggressive and fatal pleural cancer. The cell secretome offers information allowing insight into the pathogenesis of MM while offering the possibility to identify potential therapeutic targets and biomarkers. In the present study the secretome protein profile of MM cell lines was compared to normal mesothelial cells.
Six MM cell lines were compared against three primary mesothelial cell culture preparations using iTRAQ® mass spectrometry.
MM cell lines more abundantly secreted exosome-associated proteins than mesothelial cells. MM cell secretomes were enriched in proteins that are involved in response to stress, carbon metabolism, biosynthesis of amino acids, antigen processing and presentation and protein processing in the endoplasmic reticulum.
The MM cell secretome is enriched in proteins that are likely to enhance its growth and response to stress and help it inhibit an adaptive immune response. These are potential targets for therapeutic and biomarker discovery.
背景/目的：恶性间皮瘤（MM）是一种侵袭性致命性胸膜癌。细胞分泌组可提供信息，有助于深入了解MM的发病机制，同时有可能识别潜在的治疗靶点和生物标志物。在本研究中，将MM细胞系的分泌组蛋白质谱与正常间皮细胞进行了比较。
使用iTRAQ®质谱法将六种MM细胞系与三种原代间皮细胞培养制剂进行比较。
MM细胞系比间皮细胞分泌更多的外泌体相关蛋白。MM细胞分泌组富含参与应激反应、碳代谢、氨基酸生物合成、抗原加工呈递以及内质网中蛋白质加工的蛋白质。
MM细胞分泌组富含可能增强其生长和应激反应并有助于其抑制适应性免疫反应的蛋白质。这些是治疗和生物标志物发现的潜在靶点。
