Rismani Elham, Fazeli Mohammad Sadegh, Mahmoodzadeh Habibollah, Movassagh Aghdas, Azami Sina, Karimipoor Morteza, Teimoori-Toolabi Ladan
Molecular Medicine Department, Pasteur Institute of Iran, Tehran, Iran.
Department of Surgery, Division of Colo-Rectal Surgery, Imam Khomeini Medical Complex, Tehran University of Medical Sciences, Tehran, Iran.
Cancer Biomark. 2017;19(2):151-159. doi: 10.3233/CBM-160175.
Colorectal cancer (CRC) is known as one of the most leading causes of death from cancer in the world. Wnt signaling pathway is the most important signaling pathway activated in this type of cancer. The low-density lipoprotein (LDL) receptor-related protein-6 (LRP6) functions as a co-receptor in order to trigger this signaling pathway.
The expression of LRP6 was studied in cell lines, tumoral and normal adjacent tissues from patients affected with colorectal cancer.
The expression of LRP6 was assayed by quantitative Real time PCR.
LRP6 is overexpressed at the transcript and protein level in HCT116 and SW480 in comparison to HUVEC as the non-cancerous cell. Furthermore, LRP6 was significantly up-regulated in 61% of human malignant tissues (p-value = 0.0008). In inherited type of disease, this upregulation was lower than other types of colorectal cancer, while in rectal cancer the overexpression was significantly higher (p-value = 0.039). Gene expression was not correlated with the age, gender and the stage of disease.
Due to the profound overexpression of LRP6 in sporadic and rectal types of cancer compared to normal colonic ones, antagonist related approaches can be promising for targeted therapies of cancer.
结直肠癌（CRC）是世界上癌症致死的主要原因之一。Wnt信号通路是这类癌症中激活的最重要的信号通路。低密度脂蛋白（LDL）受体相关蛋白6（LRP6）作为共受体来触发该信号通路。
研究LRP6在结直肠癌患者的细胞系、肿瘤组织及正常相邻组织中的表达情况。
采用定量实时PCR检测LRP6的表达。
与非癌细胞HUVEC相比，HCT116和SW480细胞系中LRP6在转录水平和蛋白水平均过表达。此外，61%的人类恶性组织中LRP6显著上调（p值 = 0.0008）。在遗传性疾病类型中，这种上调低于其他类型的结直肠癌，而在直肠癌中过表达显著更高（p值 = 0.039）。基因表达与年龄、性别和疾病分期无关。
由于与正常结肠组织相比，LRP6在散发性和直肠型癌症中显著过表达，相关拮抗剂方法有望用于癌症的靶向治疗。
