The Epigenetic Modification of in Papillary Thyroid Carcinoma and its Effects on Clinic-Pathological Features.

BACKGROUND

Epigenetic alterations such as DNA methylation are known as the main cause of different types of cancers through inactivation of tumor suppressor genes, especially thyroid cancer. Identification of novel and effective markers are important in diagnosis and prevention of thyroid cancer. In the present study, the expression and methylation of Solute carrier family 5 member 8 () in Papillary Thyroid Carcinoma (PTC) in comparison to multinodular goiter (MNG) have been studied.

METHODS

Overall, 41 patients with PTC and 36 patients affected by MNG were recruited from four hospitals in Tehran and Qazvin, Iran in 2018. Thyroid tissues were obtained during thyroidectomy. RNA and DNA were extracted from thyroid tissues. Quantitative RT-PCR assay was performed for determining the mRNA level of while Methylation-Sensitive High-Resolution Methylation was applied for assessing the methylation status.

RESULTS

Methylation status of three regions composed of 52 CpG islands in the promoter of gene was studied by HRM assay. level in PTC tissues was significantly downregulated in average 0.4 fold in comparison with MNG tissues (=0.05). The aberrant methylation of (b) region was remarkably different in PTC and MNG cases. The promoter methylation of (c) was significantly related to mutations and vascular invasion in PTC patients.

CONCLUSION

The aberrant promoter hyper methylation of SLC5A8 was related to aggressive PTC. Therefore, there is some evidence to support the hypothesis that could be a paly important role in the development of PTC.