Lazuardi Mochamad, Hestianah Eka Pramyrtha, Restiadi Tjuk Imam
Sub-Division of Veterinary Pharmacy, Faculty of Veterinary Medicine, Universitas Airlangga, Mulyorejo Rd, 60115, Surabaya, Indonesia.
Division of Histology, Faculty of Veterinary Medicine, Universitas Airlangga, Mulyorejo Rd, 60115, Surabaya, Indonesia.
Vet World. 2023 Jun;16(6):1252-1259. doi: 10.14202/vetworld.2023.1252-1259. Epub 2023 Jun 8.
Sulfadiazine, one of the sulfonamide group's active compounds, is widely used for therapeutic production against several diseases. Veterinary drug residues can have a significant impact on human health conditions. This study aimed to develop a prototype of rapid test devices (RTDs) for detecting sulfadiazine residues on chicken carcasses based on the color indication.
Seven samples of carcasses collected from traditional breeders in Surabaya-Indonesia were prepared and tested using RTDs. This sample represents the population considering that in the last report, the use of antibiotics was more than 40%, while the ability to monitor RTDs was estimated at 100. The standard color of purple by Hex code standard color or decimal code color was used to compare the positive samples. A light-emitting diode (LED) lamp was used to observe purple color. Analysis of sulfonamides resulting from RTDs was compared using a ultraviolet-visible spectrophotometer.
Sulfonamides contamination levels of 50% and 100% were detected at concentrations of 0.472 μg/mL and 0.642 μg/mL, respectively. Sulfonamides contamination that was <0.395 μg/mL did not appear purple.
The study's findings showed that RTDs can be used to detect sulfonamides residues at a limit of detection 0.5 mg/mL after a 45 min exposure to an LED operating at a wavelength of 980 nm (p < 0.05). The limitation of RTDs was not being able to monitor the presence of residues bound in fat samples. Rapid test devices can be developed for commonly monitoring devices due to the limited technology available in the market.
磺胺嘧啶是磺胺类活性化合物之一，广泛用于多种疾病的治疗生产。兽药残留会对人类健康状况产生重大影响。本研究旨在开发一种基于颜色指示的快速检测装置（RTD）原型，用于检测鸡胴体上的磺胺嘧啶残留。
从印度尼西亚泗水的传统养殖者处收集了7份胴体样本，并使用RTD进行检测。考虑到在上一份报告中抗生素的使用超过40%，而RTD的监测能力估计为100，该样本代表了总体情况。使用十六进制代码标准颜色或十进制代码颜色的紫色标准颜色来比较阳性样本。使用发光二极管（LED）灯观察紫色。使用紫外可见分光光度计比较RTD产生的磺胺类药物分析结果。
分别在浓度为0.472μg/mL和0.642μg/mL时检测到50%和100%的磺胺类药物污染水平。浓度<0.395μg/mL的磺胺类药物污染未呈现紫色。
研究结果表明，在暴露于波长为980nm的LED 45分钟后，RTD可用于检测磺胺类药物残留，检测限为0.5mg/mL（p<0.05）。RTD的局限性在于无法监测脂肪样本中结合的残留的存在。由于市场上可用技术有限，可开发快速检测装置用于常规监测装置。
