Aikemu Ainiwaer, Amat Nurmuhamat, Yusup Abdiryim, Shan Lianlian, Qi Xinwei, Upur Halmurat
Department of Drug Analysis, Faculty of Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
Faculty of Traditional Uighur Medicine, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
Exp Ther Med. 2016 Jul;12(1):384-390. doi: 10.3892/etm.2016.3328. Epub 2016 May 10.
Abnormal Savda Munziq (ASMq), an Uighur medicine formula commonly used in the treatment of cancer, has been speculated to possess antioxidative and antiproliferative effects, and to regulate immune activity. The present study was designed to systematically elucidate the toxicity-reducing activity of ASMq in mice undergoing combination chemotherapy with doxorubicin and 5-fluorouracil (5-FU). The mice were divided into normal (saline, 10 ml/kg) and doxorubicin + 5-FU groups (doxorubicin, 2.5 mg/kg; 5-FU, 10 mg/kg on alternate days). In addition, three groups received different doses of ASMq (2, 4 and 8 g/kg), in addition to doxorubicin (2.5 mg/kg) and 5-FU (10 mg/kg) treatment on alternate days. The histology of the heart and liver, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, malondialdehyde (MDA) concentrations in heart homogenate, and various biochemical parameters of the liver were evaluated. Compared with the normal control group, ASMq dose-dependently improved a number of variables, including body weight, liver index, transaminase and total protein, and partially normalized liver and cardiac pathology. ASMq restored activities of defense antioxidant enzymes SOD and GSH-Px towards normal levels, and decreased MDA concentration in dose-dependent manner. These results demonstrated that ASMq provides significant protection against doxorubicin + 5-FU combination induced hepatotoxicity and cardiotoxicity. Further studies are required to determine the effects of ASMq against doxorubicin + 5-FU-induced toxicity during chemotherapy .
异常黑胆质成熟剂（ASMq）是维吾尔医常用的一种治疗癌症的方剂，据推测具有抗氧化、抗增殖作用，并能调节免疫活性。本研究旨在系统阐明ASMq对接受阿霉素和5-氟尿嘧啶（5-FU）联合化疗小鼠的减毒活性。将小鼠分为正常组（生理盐水，10 ml/kg）和阿霉素+5-FU组（阿霉素，2.5 mg/kg；5-FU，隔日10 mg/kg）。此外，除隔日给予阿霉素（2.5 mg/kg）和5-FU（10 mg/kg）治疗外，三组分别接受不同剂量的ASMq（2、4和8 g/kg）。评估了心脏和肝脏的组织学、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性、心脏匀浆中丙二醛（MDA）浓度以及肝脏的各种生化参数。与正常对照组相比，ASMq剂量依赖性地改善了包括体重、肝脏指数、转氨酶和总蛋白在内的多个变量，并使肝脏和心脏病理部分恢复正常。ASMq将防御抗氧化酶SOD和GSH-Px的活性恢复到正常水平，并以剂量依赖性方式降低MDA浓度。这些结果表明，ASMq对阿霉素+5-FU联合诱导的肝毒性和心脏毒性具有显著的保护作用。需要进一步研究以确定ASMq在化疗期间对阿霉素+5-FU诱导的毒性的影响。
