van Doremalen Neeltje, Falzarano Darryl, Ying Tianlei, de Wit Emmie, Bushmaker Trenton, Feldmann Friederike, Okumura Atsushi, Wang Yanping, Scott Dana P, Hanley Patrick W, Feldmann Heinz, Dimitrov Dimiter S, Munster Vincent J
Virus Ecology Unit, Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, USA.
Disease Modeling and Transmission, Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, USA.
Antiviral Res. 2017 Jul;143:30-37. doi: 10.1016/j.antiviral.2017.03.025. Epub 2017 Apr 5.
Cases of Middle East respiratory syndrome coronavirus (MERS-CoV) continue to be identified and with a lack of effective clinical treatment and no preventative strategies, treatment using convalescent plasma or monoclonal antibodies (mAbs) is a potential quick route to an intervention. Passive immunotherapy via either convalescent plasma or mAbs has proven to be effective for other infectious agents. Following infection with MERS-CoV, common marmosets were treated with high titer hyperimmune plasma or the mAb m336, at 6 and 48 h post inoculation. Both treatments reduced signs of clinical disease, but reduction in viral loads in the respiratory tract were only found in the hyperimmune plasma group. A decrease in gross pathology was found only in the mAb-treated group, but no histological differences were observed between treated and control animals. While both hyperimmune plasma and the m336 treatments reduced the severity of disease in the common marmoset, neither treatment resulted in full protection against disease.
中东呼吸综合征冠状病毒（MERS-CoV）感染病例仍在不断被发现，由于缺乏有效的临床治疗方法且没有预防策略，使用康复期血浆或单克隆抗体（mAb）进行治疗是一种潜在的快速干预途径。通过康复期血浆或单克隆抗体进行被动免疫疗法已被证明对其他传染病原体有效。在普通狨猴感染中东呼吸综合征冠状病毒后，于接种后6小时和48小时用高滴度超免疫血浆或单克隆抗体m336进行治疗。两种治疗方法都减轻了临床疾病症状，但仅在超免疫血浆组中发现呼吸道病毒载量有所降低。仅在单克隆抗体治疗组中发现大体病理学改变有所减轻，但在治疗组和对照组动物之间未观察到组织学差异。虽然超免疫血浆和m336治疗都减轻了普通狨猴疾病的严重程度，但两种治疗都未能完全预防疾病。
