Chakotiya Ankita Singh, Tanwar Ankit, Narula Alka, Sharma Rakesh Kumar
Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, Delhi 110054, India.
Department of Biotechnology, Jamia Hamdard, Delhi 110062, India.
Microb Pathog. 2017 Jun;107:254-260. doi: 10.1016/j.micpath.2017.03.029. Epub 2017 Apr 4.
Biofilm formation, low membrane permeability and efflux activity developed by Pseudomonas aeruginosa, play an important role in the mechanism of infection and antimicrobial resistance. In the present study we evaluate the antibacterial effect of Zingiber officinale against multi-drug resistant strain of P. aeruginosa. The study explores antibacterial efficacy and time-kill study concomitantly the effect of herbal extract on bacterial cell physiology with the use of flow cytometry and inhibition of biofilm formation. Z. officinale was found to inhibit the growth of P. aeruginosa, significantly. A major decline in the Colony Forming Units was observed with 3 log at 12 h of treatment. Also it is found to affect the membrane integrity of the pathogen, as 70.06 ± 2.009% cells were found to stain with Propidium iodide. In case of efflux activity 86.9 ± 2.08% cells were found in Ethidium bromide positive region. Biofilm formation inhibition ability was found in the range of 68.13 ± 4.11% to 84.86 ± 2.02%. Z.officinale is effective for killing Multi-Drug Resistant P. aeruginosa clinical isolate by affecting the cellular physiology and inhibiting the biofilm formation.
铜绿假单胞菌形成的生物膜、低膜通透性和外排活性在感染机制和抗菌耐药性中发挥着重要作用。在本研究中，我们评估了生姜对多重耐药铜绿假单胞菌菌株的抗菌作用。该研究通过流式细胞术和生物膜形成抑制作用，同时探索了草药提取物的抗菌效果和时间杀菌研究以及其对细菌细胞生理学的影响。结果发现生姜能显著抑制铜绿假单胞菌的生长。在处理12小时后，菌落形成单位出现了3个对数的大幅下降。还发现它会影响病原体的膜完整性，因为有70.06±2.009%的细胞被碘化丙啶染色。在外排活性方面，发现86.9±2.08%的细胞位于溴化乙锭阳性区域。生物膜形成抑制能力在68.13±4.11%至84.86±2.02%之间。生姜通过影响细胞生理学和抑制生物膜形成，对杀灭多重耐药铜绿假单胞菌临床分离株有效。
