野生和栽培大豆种子油含量和组成的数量性状位点分析。
Quantitative trait loci analysis of seed oil content and composition of wild and cultivated soybean.
机构信息
Key laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China.
Graduate School of Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
出版信息
BMC Plant Biol. 2020 Jan 31;20(1):51. doi: 10.1186/s12870-019-2199-7.
BACKGROUND
Soybean oil is a major source of edible oil, and the domestication of wild soybean has resulted in significant changes in oil content and composition. Extensive efforts have been made to identify genetic loci that are related to soybean oil traits. The objective of this study was to identify quantitative trait loci (QTLs) related to soybean seed oil and compare the fatty acid composition between wild and cultivated soybean.
RESULTS
Using the specific-locus amplified fragment sequencing (SLAF-seq) method, a total of 181 recombinant inbred lines (RILs) derived from a cross between wild soybean ZYD00463 (Glycine soja) and cultivated soybean WDD01514 (Glycine max) were genotyped. Finally, a high-density genetic linkage map comprising 11,398 single-nucleotide polymorphism (SNP) markers on 20 linkage groups (LGs) was constructed. Twenty-four stable QTLs for seed oil content and composition were identified by model-based composite interval mapping (CIM) across multiple environments. Among these QTLs, 23 overlapped with or were adjacent to previously reported QTLs. One QTL, qPA10_1 (5.94-9.98 Mb) on Chr. Ten is a novel locus for palmitic acid. In the intervals of stable QTLs, some interesting genes involved in lipid metabolism were detected.
CONCLUSIONS
We developed 181 RILs from a cross between wild soybean ZYD00463 and cultivated soybean WDD01514 and constructed a high-density genetic map using the SLAF-seq method. We identified 24 stable QTLs for seed oil content and compositions, which includes qPA10_1 on Chr. 10, a novel locus for palmitic acid. Some interesting genes in the QTL regions were also detected. Our study will provide useful information for scientists to learn about genetic variations in lipid metabolism between wild and cultivated soybean.
背景
大豆油是食用油的主要来源，而野生大豆的驯化导致油含量和组成发生了显著变化。人们已经做出了广泛的努力来鉴定与大豆油性状相关的遗传基因座。本研究的目的是鉴定与大豆种子油相关的数量性状基因座（QTL），并比较野生大豆和栽培大豆的脂肪酸组成。
结果
利用特异区域扩增片段测序（SLAF-seq）方法，对野生大豆 ZYD00463（Glycine soja）和栽培大豆 WDD01514（Glycine max）杂交衍生的 181 个重组自交系（RILs）进行了基因型分析。最终，构建了一个包含 20 个连锁群（LGs）的 11398 个单核苷酸多态性（SNP）标记的高密度遗传连锁图谱。通过基于模型的复合区间作图（CIM）在多个环境下共鉴定到 24 个与种子油含量和组成相关的稳定 QTL。其中 23 个 QTL 与先前报道的 QTL 重叠或相邻。第 10 号染色体上的 qPA10_1（5.94-9.98 Mb）是一个新的与棕榈酸相关的基因座。在稳定 QTL 的区间内，检测到一些与脂质代谢相关的有趣基因。
结论
我们利用 SLAF-seq 方法从野生大豆 ZYD00463 和栽培大豆 WDD01514 的杂交后代中培育了 181 个重组自交系，并构建了一个高密度遗传图谱。我们鉴定到与种子油含量和组成相关的 24 个稳定 QTL，其中包括第 10 号染色体上的 qPA10_1，这是一个与棕榈酸相关的新基因座。在 QTL 区域还检测到一些有趣的基因。本研究为科学家了解野生和栽培大豆在脂质代谢方面的遗传变异提供了有用的信息。
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