Koirala Santosh, Rao Christopher V
Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave, Urbana, IL, 61801, USA.
Methods Mol Biol. 2017;1593:73-83. doi: 10.1007/978-1-4939-6927-2_5.
Many genes are required to assemble flagella. These genes encode not only the structural elements of the flagellum but also a number of regulators that control how the flagellar genes are temporally expressed during the assembly process. These regulators also specify the likelihood that a given cell will express the flagellar genes. In particular, not all cells express the flagellar genes, resulting in mixed populations of motile and non-motile cells. Nutrients provide one signal that specifies the motile fraction. In this chapter, we describe two methods for measuring flagellar gene expression dynamics using fluorescent proteins in Salmonella enterica. Both the methods can be used to investigate the mechanisms governing flagellar gene expression dynamics.
组装鞭毛需要许多基因。这些基因不仅编码鞭毛的结构元件，还编码一些调控因子，这些调控因子控制着鞭毛基因在组装过程中如何随时间表达。这些调控因子还决定了给定细胞表达鞭毛基因的可能性。特别是，并非所有细胞都表达鞭毛基因，从而导致运动性细胞和非运动性细胞的混合群体。营养物质提供了一种决定运动性细胞比例的信号。在本章中，我们描述了两种利用荧光蛋白测量肠炎沙门氏菌中鞭毛基因表达动态的方法。这两种方法都可用于研究控制鞭毛基因表达动态的机制。
