Department of Maxillofacial Tissue Regeneration, School of Dentistry and Institute of Oral Biology, Kyung Hee University, Seoul, Republic of Korea.
J Endod. 2012 Mar;38(3):405-10. doi: 10.1016/j.joen.2011.12.025. Epub 2012 Jan 28.
We previously reported that bismuth oxide containing Portland cement (BPC) showed similar biocompatibility to Portland cement (PC) in periodontal ligament cells. However, the bioactivity of simvastatin and Emdogain (Biora AB, Malmö, Sweden) on BPC was not reported. The aim of this study was to evaluate the effects of simvastatin and Emdogain on BPC compared with mineral trioxide aggregate (MTA) in human dental pulp cells (HDPCs).
Cell growth was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) assay. Differentiation was evaluated by alkaline phosphatase (ALP) activity, alizarin red staining, and reverse-transcriptase polymerase chain reaction.
The cell growth of HDPCs exposed to Emdogain and simvastatin plus BPC was superior to those administered BPC alone and similar to those that received MTA for 14 days. The simvastatin and Emdogain groups increased the odontogenic potential of the BPC group with respect to ALP activity, mineralization nodules, messenger RNA expression of ALP, osteopontin, osteocalcin, Runx2, and osterix.
These results suggest that simvastatin and Emdogain improved cell growth and the differentiation of the BPC group in HDPCs and may be useful ingredients in BPC as pulp-capping material.
我们之前的研究表明，含氧化铋的波特兰水泥（BPC）在牙周膜细胞中与波特兰水泥（PC）具有相似的生物相容性。然而，辛伐他汀和 Emdogain（Biora AB，马尔默，瑞典）对 BPC 的生物活性尚未报道。本研究旨在评估辛伐他汀和 Emdogain 对 BPC 的影响，并与牙髓细胞（HDPCs）中的矿物三氧化物聚合体（MTA）进行比较。
通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定法来确定细胞生长情况。通过碱性磷酸酶（ALP）活性、茜素红染色和逆转录聚合酶链反应来评估分化。
暴露于 Emdogain 和辛伐他汀加 BPC 的 HDPCs 的细胞生长优于单独接受 BPC 的细胞生长，与接受 MTA 的细胞生长相似，持续 14 天。辛伐他汀和 Emdogain 组提高了 BPC 组的成牙潜能，表现为 ALP 活性、矿化结节、ALP、骨桥蛋白、骨钙素、Runx2 和osterix 的信使 RNA 表达增加。
这些结果表明，辛伐他汀和 Emdogain 可改善 HDPCs 中 BPC 组的细胞生长和分化，并且可能是 BPC 作为牙髓覆盖材料的有用成分。
