Pitzschk Monique Aparecida de Lima Rios, Bueno Carlos Eduardo da Silveira, Fontana Carlos Eduardo, Martin Alexandre Sigrist De, Rocha Daniel Guimarães Pedro, Stringheta Carolina Pessoa, Limoeiro Ana Grasiela da Silva, Braga Virgilio Marcio Bastos, Guimaraes Caroliny Chavier, Marceliano-Alves Marilia Fagury Videira, Nascimento Wayne Martins, Guimarães Thiago Sena, Pelegrine Rina Andrea
Faculdade São Leopoldo Mandic, Instituto de Pesquisa São Leopoldo Mandic, Campinas, São Paulo, Brazil.
PUC-Campinas, Center of Life Sciences, Postgraduate Program in Health Sciences, Campinas, São Paulo, Brazil.
Eur J Dent. 2025 Jul;19(3):617-623. doi: 10.1055/s-0044-1789602. Epub 2025 Mar 12.
The purpose of the study was to investigate the cytotoxicity, biocompatibility, and osteogenic effect of EndoSequence BCSealer HiFlow (BCH) and Bio-C Sealer (BCS) in osteosarcoma cells SAOS-2 compared with AH Plus Jet.
For cytotoxicity analysis, the [3-(4,5-dimethyl-thiazole)-2,5-diphenyltetrazolium bromide-MTT; Sigma/Aldrich] method was used after 24, 48, and 72 hours. For cellular bioactivity, alkaline phosphatase enzyme (ALP) was evaluated after 7 and 14 days.
MTT assay was assessed using analysis of variance (ANOVA) and Tukey's test and over time using ANOVA and Sidak's test. For ALP analysis, the one-way ANOVA and Student's paired t-tests were used.
BCS was like AH Plus Jet. After 72 hours, BCH and BCS did not differ from AH Plus Jet and showed similar behavior in terms of viability. ALP showed a difference only after 14 days. BCH had the lowest bioactivity value. AH Plus Jet and BCS were like each other.
AHP was more viable for SAOS-2, and the biocompatibility of Calcium silicate-based sealers was acceptable. The present finding assessed the biocompatibility of Calcium silicate bioceramic sealers, such as BCS, EndoSequence BCH, and AH Plus Jet, is acceptable and they have osteogenic effect and bioactivity.
本研究旨在探讨EndoSequence BCSealer HiFlow（BCH）和Bio-C封闭剂（BCS）与AH Plus Jet相比，在骨肉瘤细胞SAOS-2中的细胞毒性、生物相容性和成骨作用。
细胞毒性分析采用[3-(4,5-二甲基噻唑)-2,5-二苯基四氮唑溴盐-MTT；西格玛奥德里奇公司]法，分别于24、48和72小时后进行。细胞生物活性方面，在7天和14天后评估碱性磷酸酶（ALP）。
MTT试验采用方差分析（ANOVA）和Tukey检验进行评估，随时间变化采用ANOVA和Sidak检验。对于ALP分析，采用单因素方差分析和学生配对t检验。
BCS与AH Plus Jet相似。72小时后，BCH和BCS与AH Plus Jet无差异，在活力方面表现出相似行为。ALP仅在14天后出现差异。BCH的生物活性值最低。AH Plus Jet和BCS彼此相似。
AHP对SAOS-2更具活力，硅酸钙基封闭剂的生物相容性可接受。本研究结果评估了硅酸钙生物陶瓷封闭剂如BCS、EndoSequence BCH和AH Plus Jet的生物相容性是可接受的，且它们具有成骨作用和生物活性。
