Zhang Hong-Jian, Li Ling-Nan, Zhou Jie, Yang Qin-Qin, Liu Pei-Gang, Xu Pan, Liang Wei-Qing, Cheng Lin, Zhang Ying-Qi, Pu Jin-Bao, Hu Yi-Juan
Key Laboratory of Research and Development of Chinese Medicine of Zhejiang Province, Zhejiang Academy of Traditional Chinese Medicine, 132 Tianmushan Road, Hangzhou 310007, PR China.
Pharmacy department, Ankang people's Hospital, Jiangbei Road, Ankang 725000, PR China.
J Ethnopharmacol. 2017 May 5;203:304-311. doi: 10.1016/j.jep.2017.03.057. Epub 2017 Apr 5.
The Gnaphalium affine D. Don is used in China as a folk medicine to treat gout, anti-inflammatory, antitussive and expectorant activities. The aim of this study was to evaluate the potential of the extract of G. affine to treat hyperuricemia and acute gouty arthritis in animal model.
G. affine extract was evaluated in an experimental model with potassium oxonate (PO) induced hyperuricemia in mice which was used to evaluate anti-hyperuricemia activity and xanthine oxidase (XO) inhibition. Therapies for acute gouty arthritis was also investigated on monosodium urate (MSU) crystal induced paw edema model.
G. affine extract showed expressive results on active in reducing serum uric acid (Sur) through effect renal mGLUT9 and mURAT1 mainly and inhibit XO activity in vivo. The extract of G. affine also showed significant anti-inflammatory activity and reduced the paw swelling on MSU crystal-induced paw edema model. Meanwhile, eight major compounds were identified by HPLC-ESI-QTOF-MS/MS.
The extract of G. affine showed significant effect on evaluated models and therefore may be active agents for the treatment of hyperuricemia and acute gouty arthritis.
鼠麴草在中国作为一种民间药物用于治疗痛风、具有抗炎、镇咳和祛痰作用。本研究的目的是评估鼠麴草提取物在动物模型中治疗高尿酸血症和急性痛风性关节炎的潜力。
在草酸钠（PO）诱导的小鼠高尿酸血症实验模型中评估鼠麴草提取物，该模型用于评估抗高尿酸血症活性和黄嘌呤氧化酶（XO）抑制作用。还在尿酸钠（MSU）晶体诱导的爪肿胀模型上研究了急性痛风性关节炎的治疗方法。
鼠麴草提取物通过主要影响肾脏的mGLUT9和mURAT1降低血清尿酸（Sur），并在体内抑制XO活性，显示出显著效果。鼠麴草提取物还显示出显著的抗炎活性，并减轻了MSU晶体诱导的爪肿胀模型中的爪肿胀。同时，通过HPLC-ESI-QTOF-MS/MS鉴定了八种主要化合物。
鼠麴草提取物在评估模型中显示出显著效果，因此可能是治疗高尿酸血症和急性痛风性关节炎的活性药物。
