Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978-01, Israel.
School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK.
Trends Microbiol. 2017 Jun;25(6):430-432. doi: 10.1016/j.tim.2017.03.009. Epub 2017 Apr 6.
The majority of archaea encode CRISPR-Cas systems but only a few CRISPR-Cas-based genetic tools have been developed for organisms from this domain. Nayak and Metcalf have harnessed a bacterial Cas9 protein for genome editing in Methanosarcina acetivorans, enabling efficient gene deletion and replacement.
大多数古菌都编码 CRISPR-Cas 系统，但只有少数基于 CRISPR-Cas 的遗传工具被开发用于该领域的生物体。Nayak 和 Metcalf 利用一种细菌 Cas9 蛋白在 Methanosarcina acetivorans 中进行基因组编辑，实现了高效的基因缺失和替换。
