Radzevičiūtė Rita, Theodorou Panagiotis, Husemann Martin, Japoshvili George, Kirkitadze Giorgi, Zhusupbaeva Aigul, Paxton Robert J
Molecular Evolution and Animal Systematics, Institute of Biology, University of Leipzig, Talstraβe 33, 04103 Leipzig, Germany; General Zoology, Institute for Biology, Martin Luther University Halle-Wittenberg, Hoher Weg 8, 06120 Halle (Saale), Germany; Life Sciences Center, Vilnius University, Saulėtekio al. 7, 10223 Vilnius, Lithuania.
General Zoology, Institute for Biology, Martin Luther University Halle-Wittenberg, Hoher Weg 8, 06120 Halle (Saale), Germany; German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Deutscher Platz 5e, 04103 Leipzig, Germany.
J Invertebr Pathol. 2017 Jun;146:14-23. doi: 10.1016/j.jip.2017.04.002. Epub 2017 Apr 6.
The essential ecosystem service of pollination is provided largely by insects, which are considered threatened by diverse biotic and abiotic global change pressures. RNA viruses are one such pressure, and have risen in prominence as a major threat for honey bees (Apis mellifera) and global apiculture, as well as a risk factor for other bee species through pathogen spill-over between managed honey bees and sympatric wild pollinator communities. Yet despite their potential role in global bee decline, the prevalence of honey bee-associated RNA viruses in wild bees is poorly known from both geographic and taxonomic perspectives. We screened members of pollinator communities (honey bees, bumble bees and other wild bees belonging to four families) collected from apple orchards in Georgia, Germany and Kyrgyzstan for six common honey bee-associated RNA virus complexes encompassing nine virus targets. The Deformed wing virus complex (DWV genotypes A and B) had the highest prevalence across all localities and host species and was the only virus complex found in wild bee species belonging to all four studied families. Based on amplification of negative-strand viral RNA, we found evidence for viral replication in wild bee species of DWV-A/DWV-B (hosts: Andrena haemorrhoa and several Bombus spp.) and Black queen cell virus (hosts: Anthophora plumipes, several Bombus spp., Osmia bicornis and Xylocopa spp.). Viral amplicon sequences revealed that DWV-A and DWV-B are regionally distinct but identical in two or more bee species at any one site, suggesting virus is shared amongst sympatric bee taxa. This study demonstrates that honey bee associated RNA viruses are geographically and taxonomically widespread, likely infective in wild bee species, and shared across bee taxa.
授粉这一关键的生态系统服务主要由昆虫提供，而昆虫被认为受到各种生物和非生物全球变化压力的威胁。RNA病毒就是其中一种压力，并且已成为蜜蜂（西方蜜蜂）和全球养蜂业的主要威胁，同时也是通过管理的蜜蜂与同域野生传粉者群落之间的病原体溢出而对其他蜜蜂物种构成的风险因素。然而，尽管它们可能在全球蜜蜂数量减少中发挥作用，但从地理和分类学角度来看，与蜜蜂相关的RNA病毒在野生蜜蜂中的流行情况却鲜为人知。我们对从格鲁吉亚、德国和吉尔吉斯斯坦的苹果园中采集的传粉者群落成员（蜜蜂、熊蜂和属于四个科的其他野生蜜蜂）进行了筛查，以检测六种常见的与蜜蜂相关的RNA病毒复合体，其中包含九个病毒靶点。变形翅病毒复合体（DWV基因型A和B）在所有地点和宿主物种中的流行率最高，并且是在所研究的四个科的野生蜜蜂物种中发现的唯一病毒复合体。基于负链病毒RNA的扩增，我们发现了DWV - A/DWV - B（宿主：红腹地蜂和几种熊蜂属物种）和黑蜂王台病毒（宿主：绒足花蜂、几种熊蜂属物种、双角壁蜂和木蜂属物种）在野生蜜蜂物种中进行病毒复制的证据。病毒扩增子序列显示，DWV - A和DWV - B在区域上是不同的，但在任何一个地点的两种或更多种蜜蜂物种中是相同的，这表明病毒在同域蜜蜂分类群之间传播。这项研究表明，与蜜蜂相关的RNA病毒在地理和分类学上分布广泛，可能在野生蜜蜂物种中具有感染性，并且在蜜蜂分类群之间传播。
