Azim T, Crawford D H
Department of Haematology, Chenies Mews University College, London Medical School, UK.
Int J Cancer. 1988 Jul 15;42(1):23-8. doi: 10.1002/ijc.2910420106.
Epstein-Barr virus causes polyclonal activation and immortalization of a small percentage of peripheral blood B lymphocytes after in vitro infection. However, the susceptible B lymphocytes have not been identified. We have used the B lymphocyte activation antigen, CD23, as a marker for separating immortalized and non-immortalized Epstein-Barr virus-infected B lymphocytes and have identified the polyclonally-activated cells, using double staining for cytoplasmic immunoglobulin and viral antigens. The vast majority of cells expressing cytoplasmic immunoglobulin are negative for CD23 and for Epstein-Barr virus nuclear antigen, and are non-immortalized. Conversely, the CD23-positive, immortalized population are positive for Epstein-Barr virus nuclear antigen and negative for cytoplasmic immunoglobulin. These results define a diversity in the response of B lymphocytes to Epstein-Barr virus infection and suggest separate pathways for terminal differentiation and immortalization. This diversity may be important in determining the outcome of Epstein-Barr virus infection in humans.
体外感染后，爱泼斯坦-巴尔病毒可使一小部分外周血B淋巴细胞发生多克隆激活并永生化。然而，尚未鉴明易感性B淋巴细胞。我们使用B淋巴细胞激活抗原CD23作为标记物，以分离永生化和未永生化的爱泼斯坦-巴尔病毒感染B淋巴细胞，并通过对细胞质免疫球蛋白和病毒抗原进行双重染色来鉴定多克隆激活细胞。绝大多数表达细胞质免疫球蛋白的细胞CD23及爱泼斯坦-巴尔病毒核抗原均呈阴性，且未永生化。相反，CD23阳性的永生化细胞群爱泼斯坦-巴尔病毒核抗原呈阳性，而细胞质免疫球蛋白呈阴性。这些结果明确了B淋巴细胞对爱泼斯坦-巴尔病毒感染反应的多样性，并提示了终末分化和永生化的不同途径。这种多样性对于确定人类爱泼斯坦-巴尔病毒感染的结果可能很重要。
