Ranawat Pavitra, Bakshi Nikita
a Department of Biophysics , Panjab University, South Campus , Sector-25 , Chandigarh , India.
Toxicol Mech Methods. 2017 Jul;27(6):417-427. doi: 10.1080/15376516.2017.1296048. Epub 2017 Apr 10.
Present study analyzed the effect of naringenin, a bioflavonoid, on male reproductive function in adult mouse, after intraperitoneal treatment with varying concentrations of naringenin (2, 8 and 20 mg/kg b.wt.) for two weeks. Naringenin increased the generation of reactive oxygen species and lipid peroxidation in the testis with concomitant decrease in sperm count and motility in a dose-dependent manner. Activities of antioxidant enzymes catalase, superoxide dismutase, glutathione peroxidase and levels of reduced glutathione were found to be decreased in a dose-dependent manner. Also, the levels of oxidized glutathione were increased leading to a shift in redox ratio. Naringenin treatment also led to a dose-dependent increase in the mRNA expression of c-jun, c-fos and NF-κB. The testicular histomorphology was also altered dose dependently. Additionally, the number of apoptotic germ cells increased with increasing doses of naringenin as evident from acridine orange/ethidium bromide costaining and JC-1 staining. In conclusion, our study reveals that naringenin despite being a potent antioxidant with numerous important biological functions may also act as pro-oxidant, thus causing damaging effects in the testicular tissue.
本研究分析了生物类黄酮柚皮素对成年小鼠雄性生殖功能的影响。对成年小鼠腹腔注射不同浓度（2、8和20毫克/千克体重）的柚皮素，持续两周。柚皮素以剂量依赖的方式增加睾丸中活性氧的生成和脂质过氧化，同时精子数量和活力下降。抗氧化酶过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶的活性以及还原型谷胱甘肽水平均呈剂量依赖性降低。此外，氧化型谷胱甘肽水平升高，导致氧化还原比发生变化。柚皮素处理还导致c-jun、c-fos和NF-κB的mRNA表达呈剂量依赖性增加。睾丸组织形态学也呈剂量依赖性改变。此外，从吖啶橙/溴化乙锭共染色和JC-1染色可以明显看出，随着柚皮素剂量的增加，凋亡生殖细胞的数量也增加。总之，我们的研究表明，柚皮素尽管是一种具有众多重要生物学功能的强效抗氧化剂，但也可能作为促氧化剂，从而对睾丸组织造成损害。
