从一名未受影响的对照受试者的尿液衍生细胞中生成人类诱导多能干细胞。
Generation of human iPSCs from urine derived cells of a non-affected control subject.
作者信息
Afzal Muhammad Zeeshan, Gartz Melanie, Klyachko Ekaterina A, Khan Sadiya Sana, Shah Sanjiv J, Gupta Sweta, Shapiro Amy D, Vaughan Douglas E, Strande Jennifer L
机构信息
Department of Medicine and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States.
Department of Medicine, Northwestern University-Feinberg School of Medicine, Chicago, IL, United States.
出版信息
Stem Cell Res. 2017 Jan;18:33-36. doi: 10.1016/j.scr.2016.12.008. Epub 2016 Dec 9.
We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.
我们使用从未受影响的对照受试者收集的尿液衍生细胞（UCs），在无饲养层培养条件下生成了一条人类诱导多能干细胞（iPSC）系，用作含有纤溶酶原激活物抑制剂-1（PAI-1纯合/杂合）突变的iPSC系的对照组。编码多能山中转录因子的仙台病毒（SeV）载体以低感染复数用于重编程UCs。
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