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从患有新型杂合性纤溶酶原激活物抑制剂-1（PAI-1）突变的患者尿液来源细胞中生成人类诱导多能干细胞。

Generation of human iPSCs from urine derived cells of patient with a novel heterozygous PAI-1 mutation.

作者信息

Afzal Muhammad Zeeshan, Gartz Melanie, Klyachko Ekaterina A, Khan Sadiya Sana, Shah Sanjiv J, Gupta Sweta, Shapiro Amy D, Vaughan Douglas E, Strande Jennifer L

机构信息

Department of Medicine and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States.

Department of Medicine, Northwestern University-Feinberg School of Medicine, Chicago, IL, United States.

出版信息

Stem Cell Res. 2017 Jan;18:41-44. doi: 10.1016/j.scr.2016.12.003. Epub 2016 Dec 9.

DOI:10.1016/j.scr.2016.12.003
PMID:28395801
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5939958/
Abstract

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subjects heterozygous for a novel Plasminogen Activator Inhibitor-1 (PAI-1) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.

摘要

我们使用从携带新型纤溶酶原激活物抑制剂-1（PAI-1）突变的杂合子受试者收集的尿液衍生细胞（UCs），在无饲养层培养条件下生成了一条人诱导多能干细胞（iPSC）系。编码多能性山中转录因子的仙台病毒（SeV）载体以低感染复数用于重编程PAI-1 UCs。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c96/5939958/5d4706d8c9d2/nihms963662f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c96/5939958/9a59724964f9/nihms963662f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c96/5939958/5d4706d8c9d2/nihms963662f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c96/5939958/9a59724964f9/nihms963662f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c96/5939958/5d4706d8c9d2/nihms963662f2.jpg

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本文引用的文献

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Stem Cell Res. 2016 Nov;17(3):657-660. doi: 10.1016/j.scr.2016.11.010. Epub 2016 Nov 9.
2
The Role of Plasminogen Activator Inhibitor Type-1 in Fibrosis.1型纤溶酶原激活物抑制剂在纤维化中的作用
Semin Thromb Hemost. 2017 Mar;43(2):169-177. doi: 10.1055/s-0036-1586228. Epub 2016 Aug 24.
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PAI-1 modulates cell migration in a LRP1-dependent manner via β-catenin and ERK1/2.
外泌体通过 ERK1/2-p38/MAPK 信号通路对肌营养不良症缺失型心肌细胞发挥心脏保护作用。
Sci Rep. 2018 Nov 8;8(1):16519. doi: 10.1038/s41598-018-34879-6.
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Urine-Derived Stem Cells: The Present and the Future.尿液来源干细胞：现状与未来
Stem Cells Int. 2017;2017:4378947. doi: 10.1155/2017/4378947. Epub 2017 Nov 8.
纤溶酶原激活物抑制剂-1（PAI-1）通过β-连环蛋白和细胞外信号调节激酶1/2（ERK1/2）以低密度脂蛋白受体相关蛋白1（LRP1）依赖的方式调节细胞迁移。
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Role of plasminogen activator inhibitor-1 in senescence and aging.纤溶酶原激活物抑制剂-1在衰老和老化中的作用。
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