Hruby D E
Department of Microbiology, Oregon State University, Corvallis 97331-3804.
Clin Microbiol Rev. 1990 Apr;3(2):153-70. doi: 10.1128/CMR.3.2.153.
The development and continued refinement of techniques for the efficient insertion and expression of heterologous DNA sequences from within the genomic context of infectious vaccinia virus recombinants are among the most promising current approaches towards effective immunoprophylaxis against a variety of protozoan, viral, and bacterial human pathogens. Because of its medical relevance, this area is the subject of intense research interest and has evolved rapidly during the past several years. This review (i) provides an updated overview of the technology that exists for assembling recombinant vaccinia virus strains, (ii) discusses the advantages and disadvantages of these approaches, (iii) outlines the areas of outgoing research directed towards overcoming the limitations of current techniques, and (iv) provides some insight (i.e., speculation) about probable future refinements in the use of vaccinia virus as a vector.
在感染性痘苗病毒重组体的基因组背景下高效插入和表达异源DNA序列的技术的发展及持续完善，是目前针对多种原生动物、病毒和细菌性人类病原体进行有效免疫预防的最具前景的方法之一。由于其医学相关性，该领域是研究的热点，在过去几年中发展迅速。本综述：（i）提供了用于组装重组痘苗病毒株的现有技术的最新概述；（ii）讨论了这些方法的优缺点；（iii）概述了旨在克服现有技术局限性的未来研究领域；（iv）对痘苗病毒作为载体的可能未来改进提供了一些见解（即推测）。
