Kohara M, Abe S, Komatsu T, Tago K, Arita M, Nomoto A
Department of Microbiology, Faculty of Medicine, University of Tokyo, Japan.
J Virol. 1988 Aug;62(8):2828-35. doi: 10.1128/JVI.62.8.2828-2835.1988.
Biological tests including the monkey neurovirulence test performed on recombinants between the virulent Mahoney and attenuated Sabin 1 strains of type 1 poliovirus indicated that the genome region encoding mainly the viral capsid proteins had little correlation with the neurovirulence or attenuation phenotype of the virus. The results suggested that new vaccine strains of type 2 and type 3 polioviruses may be constructed in vitro by replacing the sequence encoding the antigenic determinants in viral capsid proteins of the Sabin 1 genome by the corresponding sequences of the type 2 and type 3 genome, respectively. Accordingly, we constructed recombinants between the Sabin 1 and Sabin 3 strains of poliovirus in which genome sequences of the Sabin 1 strain encoding most or all capsid proteins were replaced by the corresponding genome sequences of the Sabin 3 strain. One of the recombinant viruses thus constructed was fully viable and showed antigenicity and immunogenicity identical to those of type 3 poliovirus. The monkey neurovirulence tests and in vitro phenotypic marker tests (temperature sensitivity of growth, sodium bicarbonate concentration dependency of growth under agar overlay, and size of plaque) were performed on the recombinant virus. The stability of the virus in regard to the temperature sensitivity phenotype was also tested. The results suggested that the recombinant virus is a possible candidate for a new type 3 poliovirus vaccine strain.
包括对1型脊髓灰质炎病毒强毒株Mahoney和减毒株Sabin 1之间的重组体进行猴神经毒力试验在内的生物学试验表明，主要编码病毒衣壳蛋白的基因组区域与病毒的神经毒力或减毒表型几乎没有相关性。结果表明，2型和3型脊髓灰质炎病毒的新型疫苗株可能通过分别用2型和3型基因组的相应序列替换Sabin 1基因组中病毒衣壳蛋白编码抗原决定簇的序列在体外构建。因此，我们构建了脊髓灰质炎病毒Sabin 1株和Sabin 3株之间的重组体，其中Sabin 1株编码大部分或全部衣壳蛋白的基因组序列被Sabin 3株的相应基因组序列所取代。由此构建的一种重组病毒完全可行，并且显示出与3型脊髓灰质炎病毒相同的抗原性和免疫原性。对该重组病毒进行了猴神经毒力试验和体外表型标记试验（生长的温度敏感性、琼脂覆盖下生长的碳酸氢钠浓度依赖性以及蚀斑大小）。还测试了该病毒在温度敏感性表型方面的稳定性。结果表明，该重组病毒可能是新型3型脊髓灰质炎病毒疫苗株的候选毒株。
