脊髓灰质炎病毒3型leon 12 a1b的核苷酸序列：与脊髓灰质炎病毒1型的比较。
The nucleotide sequence of poliovirus type 3 leon 12 a1b: comparison with poliovirus type 1.
作者信息
Stanway G, Cann A J, Hauptmann R, Hughes P, Clarke L D, Mountford R C, Minor P D, Schild G C, Almond J W
出版信息
Nucleic Acids Res. 1983 Aug 25;11(16):5629-43. doi: 10.1093/nar/11.16.5629.
Abstract
The complete nucleotide sequence of the genome of the Sabin vaccine strain of poliovirus type 3 (P3/Leon 12 a1 b) has been determined from cDNA cloned in E. coli. The genome comprises a 5' non-coding region of 742 nucleotides, a large open reading frame of 6618 nucleotides (89% of the sequence) and a 3' non-coding region of 72 nucleotides. There is 77.4% base-sequence homology and 89.6% predicted amino-acid homology between types 1 and 3. Conservation of all glutamine-glycine and tyrosine-glycine cleavage sites suggests a mechanism of polyprotein processing similar to that established for poliovirus type 1.
摘要
脊髓灰质炎病毒3型（P3/Leon 12 a1 b）萨宾疫苗株基因组的完整核苷酸序列已通过克隆于大肠杆菌中的cDNA确定。该基因组包括一个742个核苷酸的5'非编码区、一个6618个核苷酸的大开放阅读框（序列的89%）和一个72个核苷酸的3'非编码区。1型和3型之间的碱基序列同源性为77.4%，预测的氨基酸同源性为89.6%。所有谷氨酰胺-甘氨酸和酪氨酸-甘氨酸切割位点的保守性表明，多聚蛋白加工机制与脊髓灰质炎病毒1型所确立的机制相似。
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