Research Group in Food and Human Nutrition, School of Nutrition and Dietetics, Universidad de Antioquia, Medellín, Colombia.
Independent Global Nutrition Consultant, Medellín, Colombia.
Birth Defects Res. 2017 May 1;109(8):564-573. doi: 10.1002/bdr2.1004. Epub 2017 Mar 15.
This investigation determines the nutritional state of serum and red blood cell (RBC) folate concentration and their relation with intake of folate, B ,and B , with serum vitamin B , and with genetic variants after provision of 400 μg/day of folic acid for 3 months to a group of 34 Colombian women of reproductive age.
We evaluated nutrient intake using 24-hr recall, assessing the levels of serum folate, RBC folate, serum B , and homocysteine, as well as determining genetic variants of the enzyme MTHFR (C677T and A1298C) and CβS (844ins68pb).
The results show that following intake of 400 μg/day of folic acid, the risk of folate deficiency as seen in regular dietary intake disappears and the nutritional status of this nutrient is increased (p < 0.001). With respect to vitamin B the risk of serum deficiency with folic acid consumption increased slightly, and those that were found to be B deficient after supplementation also had decreased levels of serum homocysteine. Genetic factors did not influence the nutritional status of folate, although an association was found between the intake of nutrients and biochemical indicators.
Given the results of our study, subsequent studies evaluating folic acid supplementation should also consider evaluating the status of B and B , and serum and RBC folate, as they participate interdependently in the cycle of folate and methionine and in homocysteine metabolism.Birth Defects Research 109:564-573, 2017.© 2017 Wiley Periodicals, Inc.
本研究旨在调查血清和红细胞（RBC）叶酸浓度的营养状态，以及它们与叶酸、B₁₂ 和 B₆摄入、血清维生素 B₁₂、以及摄入 400μg/天叶酸 3 个月后基因变异的关系。
我们使用 24 小时回顾法评估营养素摄入量，评估血清叶酸、RBC 叶酸、血清 B₁₂ 和同型半胱氨酸水平，并确定 MTHFR（C677T 和 A1298C）和 CβS（844ins68pb）酶的基因变异。
结果表明，摄入 400μg/天叶酸后，常规饮食摄入引起的叶酸缺乏风险消失，这种营养素的营养状况得到改善（p<0.001）。关于维生素 B，叶酸摄入后血清缺乏的风险略有增加，补充后发现 B 缺乏的人血清同型半胱氨酸水平也降低。遗传因素并未影响叶酸的营养状况，但发现营养素摄入与生化指标之间存在关联。
鉴于本研究的结果，随后评估叶酸补充的研究还应考虑评估 B₁₂ 和 B₆的状况，以及血清和 RBC 叶酸，因为它们在叶酸和蛋氨酸循环以及同型半胱氨酸代谢中相互依存。出生缺陷研究 109:564-573, 2017。© 2017 Wiley Periodicals, Inc.
