Toljic Mina, Egic Amira, Munjas Jelena, Karadzov Orlic Natasa, Milovanovic Zagorka, Radenkovic Aleksandra, Vuceljic Jovana, Joksic Ivana
Genetic Laboratory Department, Obstetrics and Gynecology Clinic "Narodni Front", Street Kraljice Natalije 62, 11000 Belgrade, Serbia.
High-Risk Pregnancy Department, Obstetrics and Gynecology Clinic "Narodni Front", Street Kraljice Natalije 62, 11000 Belgrade, Serbia; School of Medicine, University of Belgrade, Street Dr Subotica 8, 11000 Belgrade, Serbia.
Reprod Toxicol. 2017 Aug;71:55-62. doi: 10.1016/j.reprotox.2017.04.002. Epub 2017 Apr 8.
We investigated whether gestational diabetes mellitus (GDM) and gestational arterial hypertension (GH) are associated with increased oxidative stress and DNA damage. Study included 3 groups of pregnant women (GDM, GH and control). DNA damage biomarkers (micronuclei MNi, nucleoplasmic bridges NPBs and nuclear buds NBUDs) were assessed by cytokinesis-block micronucleus cytome assay. Oxidative stress levels were evaluated by analyzing malondialdehyde equivalents (TBARS) and 8-hydroxy-2'-deoxyguanosine (8-OHdG). Genotoxic effect of methyldopa, drug used to treat GH, was evaluated in in vitro experiment. TBARS levels, MNi, NPBs and NBUDs frequencies were significantly increased in both GDM and GH group. Concentrations of 8-OHdG were significantly higher in GDM than in other groups. Since methyldopa did not affect MNi, NPBs and NBUDs frequencies, nor TBARS and 8-OHdG levels, we concluded that methyldopa has no genotoxic effect. Thus, even when hyperglycemia or hypertension are present only during pregnancy they induce oxidative stress, DNA damage and chromosomal aberrations.
我们研究了妊娠期糖尿病（GDM）和妊娠期动脉高血压（GH）是否与氧化应激增加和DNA损伤有关。研究纳入了3组孕妇（GDM组、GH组和对照组）。通过胞质分裂阻滞微核细胞分析法评估DNA损伤生物标志物（微核MNi、核质桥NPBs和核芽NBUDs）。通过分析丙二醛当量（TBARS）和8-羟基-2'-脱氧鸟苷（8-OHdG）来评估氧化应激水平。在体外实验中评估了用于治疗GH的药物甲基多巴的遗传毒性作用。GDM组和GH组的TBARS水平、MNi、NPBs和NBUDs频率均显著升高。GDM组的8-OHdG浓度显著高于其他组。由于甲基多巴不影响MNi、NPBs和NBUDs频率，也不影响TBARS和8-OHdG水平，我们得出结论，甲基多巴没有遗传毒性作用。因此，即使高血糖或高血压仅在孕期出现，它们也会诱导氧化应激、DNA损伤和染色体畸变。
