Department of Chemistry and Nano-science Center, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark.
Nanoscale. 2017 May 4;9(17):5517-5527. doi: 10.1039/c6nr09700f.
Over the past decade, vertical nanostructures have provided novel approaches for biomedical applications such as intracellular delivery/detection, specific cell capture, membrane potential measurement, and cellular activity regulation. Although the feasibility of the vertical nanostructures as a new biological tool has been thoroughly demonstrated, a better understanding of cell behavior on vertical nanostructures, in particular the effects of geometry, is essential for advanced applications. To investigate the cell behavior according to the variation of the spacing between vertical nanostructures, we have interfaced fibroblasts (NIH3T3) with density-controlled vertical silicon nanocolumn arrays (vSNAs). Over a wide range of vSNA densities, we observe three distinct cell settling regimes and investigate both overall cell behavior (adhesions, morphology, and mobility) and detailed biomacromolecule variance (F-actin and focal adhesion) across these regimes. We expect that these systematic observations could serve as a guide for improved nanostructure array design for the desired cell manipulation.
在过去的十年中，垂直纳米结构为生物医学应用提供了新的方法，例如细胞内递药/检测、特定细胞捕获、膜电位测量和细胞活性调节。尽管垂直纳米结构作为一种新的生物工具的可行性已经得到了充分的证明，但是更好地理解细胞在垂直纳米结构上的行为，特别是几何形状的影响，对于高级应用至关重要。为了根据垂直纳米结构之间的间距变化来研究细胞行为，我们将成纤维细胞（NIH3T3）与密度可控的垂直硅纳米柱阵列（vSNA）进行了界面连接。在广泛的 vSNA 密度范围内，我们观察到三种不同的细胞沉降状态，并研究了这些状态下的整体细胞行为（黏附、形态和迁移）和详细的生物大分子变化（F-肌动蛋白和焦点黏附）。我们期望这些系统观察结果可以为所需的细胞操作的改进纳米结构阵列设计提供指导。
