Bionanotechnology and Nanomedicine Laboratory, Department of Chemistry and Nano-science Center, University of Copenhagen , Universitetsparken 5, DK-2100, Copenhagen, Denmark.
ACS Appl Mater Interfaces. 2013 Nov 13;5(21):10510-9. doi: 10.1021/am402070k. Epub 2013 Oct 24.
Arrays of nanowires (NWs) are currently being established as vehicles for molecule delivery and electrical- and fluorescence-based platforms in the development of biosensors. It is conceivable that NW-based biosensors can be optimized through increased understanding of how the nanotopography influences the interfaced biological material. Using state-of-the-art homogenous NW arrays allow for a systematic investigation of how the broad range of NW densities used by the community influences cells. Here it is demonstrated that indium arsenide NW arrays provide a cell-promoting surface, which affects both cell division and focal adhesion up-regulation. Furthermore, a systematic variation in NW spacing affects both the detailed cell morphology and adhesion properties, where the latter can be predicted based on changes in free-energy states using the proposed theoretical model. As the NW density influences cellular parameters, such as cell size and adhesion tightness, it will be important to take NW density into consideration in the continued development of NW-based platforms for cellular applications, such as molecule delivery and electrical measurements.
纳米线（NWs）阵列目前被用作分子输送的载体，以及在生物传感器开发中基于电和荧光的平台。可以想象，通过增加对纳米形貌如何影响界面生物材料的理解，基于 NW 的生物传感器可以得到优化。使用最先进的均匀 NW 阵列可以系统地研究社区中使用的广泛的 NW 密度如何影响细胞。在这里，证明了砷化铟 NW 阵列提供了一个促进细胞的表面，这会影响细胞分裂和粘着斑的上调。此外，NW 间距的系统变化会影响细胞的详细形态和附着特性，而后者可以根据所提出的理论模型中基于自由能状态的变化来预测。由于 NW 密度会影响细胞参数，如细胞大小和附着紧密度，因此在继续开发用于细胞应用的基于 NW 的平台时，如分子输送和电测量，考虑 NW 密度将非常重要。
