与单克隆抗体225.28S偶联的硫堇对人高分子量黑色素瘤相关抗原的体外选择性毒性
Selective in vitro toxicity of purothionin conjugated to the monoclonal antibody 225.28S to a human high-molecular-weight melanoma-associated antigen.
作者信息
Imai K, Nakanishi T, Noguchi T, Yachi A, Ferrone S
出版信息
Cancer Immunol Immunother. 1983;15(3):206-9. doi: 10.1007/BF00199166.
Abstract
The toxic agent purothionin was conjugated to the monoclonal antibody 225.28S to a human high-molecular-weight melanoma-accociated antigen. The toxic conjugate displayed in vitro toxicity to cultured human Colo 38 melanoma cells as indicated by reduced uptake of 3H-thymidine following a 24-h incubation and loss of cell viability following a 7-day incubation. The effect is dose-dependent and is specific since addition of the toxic conjugate to a cultured Raji B lymphoid cells did not affect their 3H-thymidine uptake or their viability.
摘要
毒性因子硫堇与针对人高分子量黑色素瘤相关抗原的单克隆抗体225.28S偶联。如在24小时孵育后3H-胸腺嘧啶核苷摄取减少以及在7天孵育后细胞活力丧失所示，该毒性偶联物对培养的人Colo 38黑色素瘤细胞显示出体外毒性。该效应具有剂量依赖性且具有特异性，因为将毒性偶联物添加到培养的Raji B淋巴细胞中并不影响它们的3H-胸腺嘧啶核苷摄取或活力。
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