Ondokuz Mayıs University, School of Dentistry, Department of Periodontology, Samsun, Turkey.
Ondokuz Mayıs University, Faculty of Medicine, Department of Histology and Embriology, Samsun, Turkey.
J Appl Oral Sci. 2017 Mar-Apr;25(2):121-129. doi: 10.1590/1678-77572016-0298.
This study aimed to demonstrate the effect of grape seed extract (GSE) on periodontitis.
Ligature induced periodontitis was created in 40 rats and they were assigned to four equal groups. One group was fed laboratory diet (group A) while three groups received GSE additionally. Silk ligatures were placed around the cervical area of the mandibular first molars for four weeks to induce periodontitis. The GSE groups were reallocated regarding GSE consumption as: for two weeks before ligation (group B; totally eight weeks), from ligation to two weeks after removal of the ligature (group C; totally six weeks), and for two weeks from ligature removal (group D; totally two weeks). Sections were assessed histologically and immunohistochemically. Inflammatory cell number (ICN), connective tissue attachment level (CAL), osteoclast density (OD), IL-10 and TGF-β stainings in gingival epithelium (GE), connective tissue (GC), and periodontal ligament (PL) were used as the study parameters.
Lower ICN, higher CAL, and lower OD were observed in the GSE groups (p<0.05). IL-10 was more intensive in the GSE groups and in the GEs (p<0.05). Group B showed the highest IL-10 for PL (p<0.05). TGF-ß was higher in the GEs of all groups (p<0.017).
The results suggest anti-inflammatory activities of GSE, but further investigations are needed for clarification of these activities.
本研究旨在证明葡萄籽提取物（GSE）对牙周炎的影响。
在 40 只大鼠中诱导结扎诱导性牙周炎，并将其分为四组。一组喂食实验室饮食（A 组），而三组另外喂食 GSE。用丝线结扎物环绕下颌第一磨牙的颈部区域 4 周以诱导牙周炎。根据 GSE 的消耗，将 GSE 组重新分配如下：结扎前两周（B 组；总共 8 周）、结扎至结扎去除后两周（C 组；总共 6 周）以及结扎去除后两周（D 组；总共 2 周）。对切片进行组织学和免疫组织化学评估。龈上皮（GE）、结缔组织（GC）和牙周韧带（PL）中的炎性细胞数（ICN）、结缔组织附着水平（CAL）、破骨细胞密度（OD）、IL-10 和 TGF-β染色被用作研究参数。
GSE 组的 ICN 较低，CAL 较高，OD 较低（p<0.05）。GSE 组和 GE 中的 IL-10 更强烈（p<0.05）。B 组显示 PL 中 IL-10 最高（p<0.05）。所有组的 GE 中的 TGF-β 更高（p<0.017）。
这些结果表明 GSE 具有抗炎活性，但需要进一步研究以阐明这些活性。
