Department of Oral and Maxillofacial Surgery, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Department of Pathology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania.
J Med Life. 2020 Oct-Dec;13(4):580-586. doi: 10.25122/jml-2020-0177.
The associations between periodontitis and cardiovascular diseases have been intensely studied in recent years. Oxidative stress is involved in the initiation and both progression of periodontitis and atherosclerosis. Antioxidants can reduce the effects of oxidative stress on inflammatory diseases. Our aim was to measure the effects of a grape seed extract (GSE), rich in antioxidants, on atherosclerosis caused by ligature-induced periodontitis in rats. Thirty male Wistar rats were randomly divided into three groups of 10: control group, periodontitis group, and periodontitis group treated with GSE (GSE group). Periodontitis was induced by placing an orthodontic wire around the cervix of the first mandibular molar and keeping it in place for 4 weeks. On days 1, 7 and 28, blood samples were taken to assess oxidative stress and inflammation markers (malondialdehyde and glutathione - MDA, reduced glutathione - GSH, C reactive protein) and lipids. After 4 weeks, the animals were euthanized, and aortas were collected for histopathologic examination. MDA was significantly higher in Periodontitis group compared to the other groups only at day 7. GSH was significantly increased in the Control and GSE groups on days 1 and 7, compared to Periodontitis group and on day 28 higher in GSE vs. Periodontitis groups. C reactive protein was significantly increased in the Periodontitis group on days 1 and 7 compared to both groups. Cholesterol was significantly decreased in the aortas of GSE group at day 28 compared to the Periodontitis group. Oral administration of a grape seed extract reduces the oxidative stress, inflammation and atherosclerosis in a rat model of ligature-induced periodontitis.
近年来，牙周炎与心血管疾病之间的关联已成为研究热点。氧化应激参与了牙周炎和动脉粥样硬化的发生和发展。抗氧化剂可以减轻氧化应激对炎症性疾病的影响。我们的目的是测量富含抗氧化剂的葡萄籽提取物（GSE）对结扎诱导的牙周炎大鼠动脉粥样硬化的影响。30 只雄性 Wistar 大鼠被随机分为三组，每组 10 只：对照组、牙周炎组和牙周炎组用 GSE 治疗（GSE 组）。通过在第一下颌磨牙颈周围放置正畸丝并保持 4 周来诱导牙周炎。在第 1、7 和 28 天，采集血样以评估氧化应激和炎症标志物（丙二醛和谷胱甘肽-MDA、还原型谷胱甘肽-GSH、C 反应蛋白）和脂质。4 周后，处死动物，收集主动脉进行组织病理学检查。与其他组相比，牙周炎组在第 7 天的 MDA 显著升高。与牙周炎组相比，对照组和 GSE 组在第 1 天和第 7 天的 GSH 显著增加，而 GSE 组在第 28 天的 GSH 则高于牙周炎组。与对照组和 GSE 组相比，牙周炎组在第 1 天和第 7 天的 C 反应蛋白显著升高。与牙周炎组相比，GSE 组在第 28 天的主动脉胆固醇显著降低。口服葡萄籽提取物可减轻结扎诱导的牙周炎大鼠模型中的氧化应激、炎症和动脉粥样硬化。
