Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria - Centro di Ricerca per l'Agrobiologia e la Pedologia (CREA-ABP), via di Lanciola 12/A, 50125, Firenze, Italy.
Department of Biology, LEMM, Laboratory of Microbial and Molecular Evolution Florence, University of Florence, I-50019, Sesto Fiorentino (FI), Italy.
Sci Rep. 2017 Apr 12;7(1):839. doi: 10.1038/s41598-017-00876-4.
Cold environments dominate Earth's biosphere, hosting complex microbial communities with the ability to thrive at low temperatures. However, the underlying molecular mechanisms and the metabolic pathways involved in bacterial cold-adaptation mechanisms are still not fully understood. Herein, we assessed the metabolic features of the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 (PhTAC125), a model organism for cold-adaptation, at both 4 °C and 15 °C, by integrating genomic and phenomic (high-throughput phenotyping) data and comparing the obtained results to the taxonomically related Antarctic bacterium Pseudoalteromonas sp. TB41 (PspTB41). Although the genome size of PspTB41 is considerably larger than PhTAC125, the higher number of genes did not reflect any higher metabolic versatility at 4 °C as compared to PhTAC125. Remarkably, protein S-thiolation regulated by glutathione and glutathionylspermidine appeared to be a new possible mechanism for cold adaptation in PhTAC125. More in general, this study represents an example of how 'multi-omic' information might potentially contribute in filling the gap between genotypic and phenotypic features related to cold-adaptation mechanisms in bacteria.
寒冷环境主导着地球的生物圈，拥有能够在低温下茁壮成长的复杂微生物群落。然而，细菌适应寒冷的潜在分子机制和代谢途径仍未完全被理解。在此，我们通过整合基因组和表型（高通量表型分析）数据，评估了南极细菌假交替单胞菌 TAC125（PhTAC125）的代谢特征，该菌是适应寒冷的模式生物，分别在 4°C 和 15°C 下进行研究，并将获得的结果与分类上相关的南极细菌假交替单胞菌 TB41（PspTB41）进行比较。尽管 PspTB41 的基因组大小明显大于 PhTAC125，但与 PhTAC125 相比，更多的基因并没有反映出在 4°C 时更高的代谢多样性。值得注意的是，由谷胱甘肽和谷胱甘肽亚精胺调节的蛋白质 S-巯基化似乎是 PhTAC125 适应寒冷的新可能机制。更普遍地说，这项研究代表了一种如何利用“多组学”信息来填补与细菌适应寒冷机制相关的基因型和表型特征之间差距的范例。
