感染……的儿童和青少年中白细胞介素8（-251 T>A）基因多态性
Interleukin 8 (-251 T>A) polymorphism in children and teenagers infected with .
作者信息
Saes Marina, de Labio Roger Willian, Rasmussen Lucas Trevizani, Payão Spencer Luiz Marques
机构信息
Faculdade de Medicina de Marília (FAMEMA), rua Lourival Freire, 240, Jardim Fragata, CEP 17519-050 Marília, SP Brazil.
Universidade do Sagrado Coração, Bauru, SP Brazil.
出版信息
J Venom Anim Toxins Incl Trop Dis. 2017 Apr 8;23:23. doi: 10.1186/s40409-017-0113-z. eCollection 2017.
BACKGROUND
() is a gram-negative bacterium that colonizes the human stomach and causes a variety of gastric diseases. This study evaluated the correlations between the -251 (T>A) (rs4073) polymorphism of interleukin-8 (IL-8), the etiology of gastric disease, and infection in pediatric and adolescent patients.
METHODS
DNA samples were obtained from 285 gastric biopsies from pediatric patients. was detected by PCR, whereas PCR-RFLP was used to characterize the -251 (T>A) polymorphism of IL-8.
RESULTS
The histological analysis revealed the presence of gastritis in 158 patients (55.44%). was found in 71 samples (24.9%). The -251 (T>A) polymorphism revealed that 58 (29.47%) samples were TT, 143 (50.18%) samples were TA, and 84 (20.35%) samples were AA.
CONCLUSIONS
Our findings suggest that IL8-251 A allele may be an important risk factor for the development of gastric disease when associated with infection.
背景
（）是一种革兰氏阴性菌，可定植于人的胃部并引发多种胃部疾病。本研究评估了儿科和青少年患者中白细胞介素-8（IL-8）-251（T>A）（rs4073）多态性、胃部疾病病因与感染之间的相关性。
方法
从儿科患者的285份胃活检样本中获取DNA样本。通过聚合酶链反应（PCR）检测（此处原文缺失具体检测对象），而采用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）来鉴定IL-8的-251（T>A）多态性。
结果
组织学分析显示158例患者（55.44%）存在胃炎。在71份样本（24.9%）中发现（此处原文缺失具体发现对象）。-251（T>A）多态性分析显示，58份样本（29.47%）为TT型，143份样本（50.18%）为TA型，84份样本（20.35%）为AA型。
结论
我们的研究结果表明，IL8 - 251 A等位基因在与感染相关时，可能是胃部疾病发生的一个重要风险因素。
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