Pagalilauan Hera Angela M, Paraoan Cielo Emar M, Vital Pierangeli G
a Institute of Biology, College of Science , University of the Philippines , Diliman, Quezon City , Philippines.
b Natural Sciences Research Institute , University of the Philippines , Diliman, Quezon City , Philippines.
Arch Environ Occup Health. 2018 Mar 4;73(2):107-114. doi: 10.1080/19338244.2017.1299087. Epub 2017 Apr 18.
Payatas landfill site is one of the major dumpsites in the Philippines. In this study, the landfill was evaluated regarding the presence and concentration of pathogenic bacterial aerosols through culture and molecular techniques. Bioaerosols are biological airborne particles that may or may not cause diseases. Active sampling was done using MAS-100 Air Sampler from both indoor and outdoor locations. To test the accuracy of the culture method, those that were presumptively identified as Staphylococcus aureus and Klebsiella pneumoniae were subjected to molecular analysis using 16s rRNA sequencing, and as a result, they were identified as different species. The exposure assessment done by interview showed matches among prevalent diseases. The results of this study can be used as a basis for creating standards and regulations for workers' and residents' safety and health in the landfill site.
帕亚塔斯垃圾填埋场是菲律宾主要的垃圾处理场之一。在本研究中，通过培养和分子技术对该垃圾填埋场中致病细菌气溶胶的存在情况和浓度进行了评估。生物气溶胶是可能致病或不致病的空气传播生物颗粒。使用MAS - 100空气采样器在室内和室外地点进行了主动采样。为了测试培养方法的准确性，对那些初步鉴定为金黄色葡萄球菌和肺炎克雷伯菌的样本进行了16s rRNA测序的分子分析，结果发现它们属于不同的物种。通过访谈进行的暴露评估显示了常见疾病之间的匹配情况。本研究结果可作为制定垃圾填埋场工人和居民安全与健康标准及法规的依据。
