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Pulsed-field electrophoresis indicates larger-than-expected sizes for mycoplasma genomes.

作者信息

Pyle L E, Corcoran L N, Cocks B G, Bergemann A D, Whitley J C, Finch L R

机构信息

Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.

出版信息

Nucleic Acids Res. 1988 Jul 11;16(13):6015-25. doi: 10.1093/nar/16.13.6015.

DOI:10.1093/nar/16.13.6015
PMID:2840639
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC336844/
Abstract

The sizes of large DNA fragments produced from genomes of members of the Mycoplasmataceae by digestion with restriction endonucleases having infrequent (1 to 3) cleavage sites within the genome were estimated from their mobility in contour-clamped homogeneous electric field (CHEF) agarose gel electrophoresis by comparison with yeast chromosomal DNA markers. The estimates of total genome size for 7 strains of 6 species ranged from approximately 900 kilo base pairs (kb) for Ureaplasma urealyticum 960T to 1330 kb for M. mycoides subsp. mycoides, GC-1176. The values derived from this new method are considerably higher than those of approximately 500 Mdaltons or 750 kb previously reported for genome sizes in members of the Mycoplasmataceae.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7a0/336844/9e04b95a9b26/nar00156-0316-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7a0/336844/46e7128a13a3/nar00156-0312-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7a0/336844/22d360d54e6a/nar00156-0314-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7a0/336844/9e04b95a9b26/nar00156-0316-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7a0/336844/46e7128a13a3/nar00156-0312-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7a0/336844/22d360d54e6a/nar00156-0314-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7a0/336844/9e04b95a9b26/nar00156-0316-a.jpg

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本文引用的文献

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Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.通过正交交变电场凝胶电泳从酵母中分离染色体DNA分子。
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Localizing the replication origin region on the physical map of the Mycoplasma capricolum genome.在山羊支原体基因组物理图谱上定位复制起始区域。
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Mapping of replication initiation site in Mycoplasma capricolum genome by two-dimensional gel-electrophoretic analysis.通过二维凝胶电泳分析绘制山羊支原体基因组中的复制起始位点图谱。
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In vivo damage and recA-dependent repair of plasmid and chromosomal DNA in the radiation-resistant bacterium Deinococcus radiodurans.抗辐射细菌耐辐射球菌中质粒和染色体DNA的体内损伤及RecA依赖性修复
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Characterization of the mycoplasma genome.支原体基因组的特征描述。
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