具有一些功能基因座的蕈状支原体丝状亚种Y基因组物理图谱。
A physical map of the genome of Mycoplasma mycoides subspecies mycoides Y with some functional loci.
作者信息
Pyle L E, Finch L R
机构信息
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
出版信息
Nucleic Acids Res. 1988 Jul 11;16(13):6027-39. doi: 10.1093/nar/16.13.6027.
Abstract
A physical map is presented for the 1200 kb genome of Mycoplasma mycoides subsp. mycoides Y, locating 32 cleavage sites for 8 restriction endonucleases. The large restriction fragments involved were separated and sized by pulsed-field agarose gel electrophoresis. Their locations on the map were determined by probing Southern blots of digests with individual fragments isolated from other digests and by correlating the products of double and triple digestions. Loci for 2 ribosomal RNA operons and 2 tRNA operons have been determined by probing with cloned genes and the broad regions of the replication origin and terminus have also been outlined by in vivo labelling studies.
摘要
本文展示了丝状支原体丝状亚种Y株1200 kb基因组的物理图谱，确定了8种限制性内切酶的32个切割位点。通过脉冲场琼脂糖凝胶电泳分离并测定了所涉及的大限制性片段的大小。通过用从其他酶切消化物中分离出的单个片段探测酶切消化物的Southern杂交印迹，并通过对比双酶切和三酶切产物，确定了这些片段在图谱上的位置。通过用克隆基因进行探测，确定了2个核糖体RNA操纵子和2个tRNA操纵子的基因座，并且通过体内标记研究也勾勒出了复制起点和终点的大致区域。
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本文引用的文献
1
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J Bacteriol. 1983 Apr;154(1):17-22. doi: 10.1128/jb.154.1.17-22.1983.
2
Sequence analysis of a cluster of twenty-one tRNA genes in Bacillus subtilis.枯草芽孢杆菌中一组二十一个tRNA基因的序列分析。
Nucleic Acids Res. 1983 Aug 25;11(16):5763-74. doi: 10.1093/nar/11.16.5763.
3
Ribosomal RNA genes in Mycoplasma.支原体中的核糖体RNA基因。
Nucleic Acids Res. 1982 Jul 24;10(14):4215-22. doi: 10.1093/nar/10.14.4215.
4
Mycoplasmas (Mollicutes) have a low number of rRNA genes.支原体（柔膜菌纲）的核糖体RNA基因数量较少。
J Bacteriol. 1984 Apr;158(1):376-8. doi: 10.1128/jb.158.1.376-378.1984.
5
Evidence for the bidirectional replications of the Escherichia coli chromosome.大肠杆菌染色体双向复制的证据。
Nat New Biol. 1971 Aug 4;232(31):137-40. doi: 10.1038/newbio232137a0.
6
Origin and sequence of chromosome replication in Escherichia coli.大肠杆菌中染色体复制的起源与顺序
J Mol Biol. 1972 Oct 14;70(3):549-66. doi: 10.1016/0022-2836(72)90559-1.
7
Rapid transfer of DNA from agarose gels to nylon membranes.DNA从琼脂糖凝胶快速转移至尼龙膜。
Nucleic Acids Res. 1985 Oct 25;13(20):7207-21. doi: 10.1093/nar/13.20.7207.
8
Electrophoretic separations of large DNA molecules by periodic inversion of the electric field.通过电场的周期性反转对大型DNA分子进行电泳分离。
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9
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10
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Nucleic Acids Res. 1987 Jan 26;15(2):857. doi: 10.1093/nar/15.2.857.
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