Petzel-Witt Silvana, Pogoda Werner, Wunder Cora, Paulke Alexander, Schubert-Zsilavecz Manfred, Toennes Stefan W
Institute of Legal Medicine, Goethe-University, Frankfurt, Kennedyallee 104, D-60596, Frankfurt/Main, Germany.
Institute for Pharmaceutical Chemistry, Goethe-University Frankfurt, Max-von-Laue-Str. 9, D-60438, Frankfurt/Main, Germany.
Drug Test Anal. 2018 Jan;10(1):177-183. doi: 10.1002/dta.2206. Epub 2017 Jun 22.
Ethyl glucuronide (EtG) is increasingly used in forensic toxicology as a marker for alcohol use in analyses of hair samples, especially in abstinence control. Some cosmetic treatments are considered to markedly reduce the EtG content. In view of especially many women with coloured hair the present study was performed to further investigate the effect of a variety of colouring procedures (bleaching, tinting, permanent and semi-permanent dyeing, henna) on the EtG content. Untreated hair samples (n = 12, EtG 13.9-64.7 pg/mg) were re-analyzed (gas chromatography- negative chemical ionization mass spectrometry, 0.8 pg/mg quantification limit) after different treatment procedures. A decrease of the EtG content of at least 10% occurred in every case. The reduction in comparison to the untreated hair was expectedly high for permanent dyeing and bleaching with 18.1% of the initial content (median, range 0.0-50.9%) and 18.4% (0.0-46.7%), respectively. For henna this was 38.3% (0.0-83.0%), for tinting 70.4% (29.0-90.8%), for semi-permanent dyeing 41.9% (0.0-77.4%). With permanent hair dye the EtG content was decreased to below 7 pg/mg in 10 of 12 cases, in 3 cases even below the LOD (0.2 pg/mg). Surprisingly henna treatment without oxidative component had a marked influence, EtG was below 2 pg/mg in 2 of 12 samples. The study showed that all tested coloration procedures markedly affected the deposited EtG content. Even temporary or henna coloration may have a marked effect. The present data support the recommendation to exclude hair samples with colour manipulations for analysis on the EtG content as a precaution in alcohol abstinence programs. Copyright © 2017 John Wiley & Sons, Ltd.
乙基葡萄糖醛酸苷（EtG）在法医毒理学中越来越多地被用作头发样本酒精使用情况分析的标志物，尤其是在戒酒监测方面。一些美容治疗被认为会显著降低EtG含量。鉴于有特别多的女性染有彩色头发，本研究旨在进一步调查各种染发程序（漂白、染发、永久性和半永久性染发、指甲花染发）对EtG含量的影响。在不同处理程序后，对未处理的头发样本（n = 12，EtG含量为13.9 - 64.7 pg/mg）进行重新分析（气相色谱 - 负化学电离质谱法，定量限为0.8 pg/mg）。每种情况下EtG含量至少降低了10%。与未处理的头发相比，永久性染发和漂白的EtG含量降低幅度预期较高，分别为初始含量的18.1%（中位数，范围0.0 - 50.9%）和18.4%（0.0 - 46.7%）。指甲花染发的降低幅度为38.3%（0.0 - 83.0%），染发为70.4%（29.0 - 90.8%），半永久性染发为41.9%（0.0 - 77.4%）。使用永久性染发剂时，12个案例中有10个案例的EtG含量降至7 pg/mg以下，3个案例甚至低于检测限（0.2 pg/mg）。令人惊讶的是，不含氧化成分的指甲花染发处理有显著影响，12个样本中有2个样本的EtG含量低于2 pg/mg。该研究表明，所有测试的染发程序均显著影响沉积的EtG含量。即使是临时染发或指甲花染发也可能有显著影响。目前的数据支持在戒酒项目中作为预防措施排除经过颜色处理的头发样本用于EtG含量分析的建议。版权所有© 2017约翰威立父子有限公司。
