Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
J Hazard Mater. 2017 Jul 15;334:121-131. doi: 10.1016/j.jhazmat.2017.03.055. Epub 2017 Mar 27.
Water environment pollution caused by the widespread application of beta-cypermethrin (BCP) and chlorpyrifos (CPF) in agriculture has attracted extensive concern of the world. In this study, zebrafish was used as a model to investigate the individual and joint toxicity of BCP and CPF. In the acute toxicity test, 3 hpf embryos were exposed to various concentrations of CPF, BCP and their binary mixtures (MIX) for 96h. The results indicated that these two pesticides and mixtures induced malformation and death in larvae, and affected hatchability. These two pesticides in mixtures were verified to act together in a synergistic manner under experimental conditions. Oxidative stress assaying manifested that CPF, BCP and MIX altered CAT, SOD and GST activities and MDA content, resulting in oxidative damage in larvae. By pathology analysis, CPF (236μg/L), BCP (5.9μg/L) and MIX (236μg/L CPF+5.9μg/L BCP) were found to trigger liver lesions and promote apoptosis in tissues. The transcriptome sequencing suggested that ECM- receptor interaction, focal adhesion, cell cycle, DNA replication, phototransduction and adherens junction pathways were closely associated with the toxicity of these two pesticides.
水环境受农业上广泛应用的氯氰菊酯（CPF）和高效氯氰菊酯（BCP）污染引起了世界广泛关注。本研究以斑马鱼为模型，研究了 BCP 和 CPF 的单独及联合毒性。在急性毒性试验中，将 3 hpf 胚胎暴露于不同浓度的 CPF、BCP 及其二元混合物（MIX）中 96h。结果表明，这两种农药及其混合物在实验条件下协同作用，导致幼虫畸形和死亡，并影响孵化率。氧化应激测定表明 CPF、BCP 和 MIX 改变了 CAT、SOD 和 GST 活性以及 MDA 含量，导致幼虫氧化损伤。通过病理学分析发现，CPF（236μg/L）、BCP（5.9μg/L）和 MIX（236μg/L CPF+5.9μg/L BCP）引发肝脏病变并促进组织细胞凋亡。转录组测序表明，ECM-受体相互作用、黏附斑、细胞周期、DNA 复制、光转导和黏着连接途径与这两种农药的毒性密切相关。
