Friebe-Hoffmann Ulrike, Antony Linda, Kruessel Jan-Steffen, Pawlowski Brigitte, Hoffmann Thomas Karl
Department of Obstetrics & Gynecology, University of Ulm, Ulm, Germany.
Department of Radiation Oncology, Marienhospital, Duesseldorf, Germany.
Exp Clin Endocrinol Diabetes. 2017 Nov;125(10):677-683. doi: 10.1055/s-0043-104935. Epub 2017 Apr 13.
During the last decades the incidence of diabetes has dramatically increased as well as the number of pregnant diabetic women. There is still missing data regarding patterns and shifts of immune cell populations due to pregnancy with or without diabetes. The study aimed to investigate the impact of pregnancy, type 1 diabetes (T1D) and gestational diabetes mellitus (GDM) on different immune cells in female. The number and proportion of CD3-, CD4-, CD8- and γδ T-cells as well as B-, NK-, NKT- and dendritic cells (DC) incl. rate of apoptosis was analyzed in peripheral blood samples from 24 non-pregnant women, 24 pregnant controls, 25 non-pregnant T1D, 18 women with GDM and 15 pregnant T1D (PT1D) women. Compared to healthy controls, healthy pregnant women had reduced numbers of lymphoid DC and γδ T-cells, while women with gestational diabetes presented with increased numbers of γδ T-cells. Pregnant women with T1D showed increased NKT cells and a decrease of NK cells compared to healthy pregnant or non-pregnant T1D women. Apoptosis of γδ T-cells in healthy pregnant women was found to be decreased in comparison to their non-pregnant controls while apoptosis of myeloid and lymphoid DC was increased in pregnant T1D in comparison to non-pregnant T1D. Those results may indicate that increased complication rates during diabetic pregnancies might be due to an impaired adaptation of the immune system.
在过去几十年中，糖尿病的发病率以及妊娠糖尿病女性的数量都急剧增加。关于妊娠（无论是否患有糖尿病）导致的免疫细胞群体模式和变化，仍然缺乏数据。该研究旨在调查妊娠、1型糖尿病（T1D）和妊娠期糖尿病（GDM）对女性不同免疫细胞的影响。分析了24名未怀孕女性、24名怀孕对照者、25名未怀孕的T1D患者、18名GDM女性和15名怀孕的T1D（PT1D）女性外周血样本中CD3、CD4、CD8和γδ T细胞以及B细胞、NK细胞、NKT细胞和树突状细胞（DC）的数量和比例，包括凋亡率。与健康对照相比，健康孕妇的淋巴样DC和γδ T细胞数量减少，而妊娠期糖尿病女性的γδ T细胞数量增加。与健康孕妇或未怀孕的T1D女性相比，患有T1D的孕妇显示NKT细胞增加，NK细胞减少。发现健康孕妇中γδ T细胞的凋亡与其未怀孕对照相比减少，而与未怀孕的T1D相比，怀孕的T1D中髓样和淋巴样DC的凋亡增加。这些结果可能表明，糖尿病妊娠期间并发症发生率增加可能是由于免疫系统适应性受损。
