Laboratory of Microbiome Immunobiology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 12 R. Weigla Str., 53-114 Wrocław, Poland.
Int J Mol Sci. 2022 Mar 14;23(6):3125. doi: 10.3390/ijms23063125.
Ageing is accompanied by the inevitable changes in the function of the immune system. It provides increased susceptibility to chronic infections that have a negative impact on the quality of life of older people. Therefore, rejuvenating the aged immunity has become an important research and therapeutic goal. Yolkin, a polypeptide complex isolated from hen egg yolks, possesses immunoregulatory and neuroprotective activity. Considering that macrophages play a key role in pathogen recognition and antigen presentation, we evaluated the impact of yolkin on the phenotype and function of mouse bone marrow-derived macrophages of the BMDM cell line. We determined yolkin bioavailability and the surface co-expression of CD80/CD86 using flow cytometry and IL-6, IL-10, TGF-β and iNOS mRNA expression via real-time PCR. Additionally, the impact of yolkin on the regulation of cytokine expression by MAPK and PI3K/Akt kinases was determined. The stimulation of cells with yolkin induced significant changes in cell morphology and an increase in CD80/CD86 expression. Using pharmaceutical inhibitors of ERK, JNK and PI3K/Akt, we have shown that yolkin is able to activate these kinases to control cytokine mRNA expression. Our results suggest that yolkin is a good regulator of macrophage activity, priming mainly the M1 phenotype. Therefore, it is believed that yolkin possesses significant therapeutic potential and represents a promising possibility for the development of novel immunomodulatory medicine.
衰老是伴随着免疫系统功能不可避免的变化而发生的。它增加了对慢性感染的易感性，对老年人的生活质量产生负面影响。因此，恢复衰老的免疫力已成为一个重要的研究和治疗目标。Yolkin 是一种从鸡蛋蛋黄中分离出来的多肽复合物，具有免疫调节和神经保护活性。鉴于巨噬细胞在病原体识别和抗原呈递中发挥关键作用，我们评估了 yolkin 对 BMDM 细胞系的小鼠骨髓来源巨噬细胞（BMDM）的表型和功能的影响。我们使用流式细胞术确定 yolkin 的生物利用度和 CD80/CD86 的表面共表达，并通过实时 PCR 确定 IL-6、IL-10、TGF-β 和 iNOS mRNA 表达。此外，还确定了 yolkin 对 MAPK 和 PI3K/Akt 激酶调节细胞因子表达的影响。yolkin 刺激细胞会引起细胞形态的显著变化，并增加 CD80/CD86 的表达。使用 ERK、JNK 和 PI3K/Akt 的药物抑制剂，我们表明 yolkin 能够激活这些激酶来控制细胞因子 mRNA 的表达。我们的结果表明 yolkin 是一种很好的巨噬细胞活性调节剂，主要诱导 M1 表型。因此，人们认为 yolkin 具有显著的治疗潜力，代表了开发新型免疫调节药物的有希望的可能性。
