Blok J, Kay B H, Hall R A, Gorman B M
Queensland Institute of Medical Research, Herston, Brisbane, Australia.
Arch Virol. 1988;100(3-4):213-20. doi: 10.1007/BF01487684.
Thirteen strains of dengue type 1 were isolated from the lymphocyte fractions of 69 acute phase blood samples collected at Thursday Island Hospital during 1981 and 1982. One further strain of type 1 was isolated from 7 blood samples despatched by air from Cairns Base Hospital during 1982. Four of these Australian isolates representing the beginning, middle, and end of the epidemic were examined by restriction enzyme mapping and were found to be identical for the nine restriction enzymes used. The maps differed from those derived from two Malaysian dengue type 1 strains isolated during the epidemic of 1981-82 in that country. This suggests reliance on serological typing to establish global circulation patterns of epidemic dengue is insufficient and that more specific methods such as genome mapping are useful.
1981年至1982年期间，从星期四岛医院采集的69份急性期血样的淋巴细胞组分中分离出13株1型登革病毒。1982年，又从凯恩斯基地医院空运来的7份血样中分离出1株1型病毒。对其中代表该流行病起始、中期和末期的4株澳大利亚分离株进行了限制性酶切图谱分析，发现所用的9种限制性酶的图谱完全相同。这些图谱与从该国1981 - 1982年流行期间分离出的两株马来西亚1型登革病毒的图谱不同。这表明依靠血清学分型来确定流行登革热的全球传播模式是不够的，而基因组图谱等更特异的方法是有用的。
