Blok J, Henchal E A, Gorman B M
J Gen Virol. 1984 Dec;65 ( Pt 12):2173-81. doi: 10.1099/0022-1317-65-12-2173.
Variable amounts of cDNA were synthesized in vitro from RNA extracted from several flaviviruses, including the four prototype dengue (DEN) virus serotypes. The synthesis was carried out using an oligo(dT) primer, suggesting the presence of a short poly(A) region at or near the 3' end of some flavivirus genomes. The DEN-1 and DEN-2 prototype strains produced the largest amount of cDNA and were therefore used to investigate further the relatedness of flavivirus genomes by cDNA-RNA hybridization. The flaviviruses studied are related to each other to some extent since the hybrids formed exhibited about 30% S1 nuclease resistance, but a closer relationship was detected between dengue viruses of serotype 1 and 4 and between dengue virus serotype 2 and Edge Hill virus. A monoclonal antibody to the envelope protein (V3) of dengue viruses reacted with Edge Hill virus, confirming the genetic relationship between the viruses.
从包括四种登革热（DEN）病毒原型血清型在内的几种黄病毒提取的RNA体外合成了不同量的互补DNA（cDNA）。合成过程使用寡聚（dT）引物，这表明某些黄病毒基因组3'端或其附近存在短聚腺苷酸（poly(A)）区域。DEN-1和DEN-2原型毒株产生的cDNA量最大，因此被用于通过cDNA-RNA杂交进一步研究黄病毒基因组的相关性。所研究的黄病毒在一定程度上彼此相关，因为形成的杂交体表现出约30%的S1核酸酶抗性，但在血清型1和4的登革病毒之间以及血清型2的登革病毒和埃奇希尔病毒之间检测到更密切的关系。一种针对登革病毒包膜蛋白（V3）的单克隆抗体与埃奇希尔病毒发生反应，证实了这些病毒之间的遗传关系。
